Other Immunogen Type corresponding to Chicken Progesterone Receptor. Progesterone receptor purified from chick oviduct cytosol.
Our Abpromise guarantee covers the use of ab2765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 20 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 99 kDa.|
|ChIP||Use at an assay dependent concentration.|
|EMSA||Use at an assay dependent concentration.|
|Flow Cyt||Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on human uterus tissue. Antigen retrieval was performed using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2765 (1:20) overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
Immunocytochemistry/ Immunofluorescence analysis of T47D cells untreated (left) or stimulated with 100nm promegestone for 1 hour (right), labeling Progesterone Receptor with ab2765 (green). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA for 15 minutes at room temperature. Cells were stained with Anti-Progesterone Receptor antibody [Alpha PR6] - ChIP Grade (ab2765) at a dilution of 1/100 for 1 hour at 37C, and then incubated with a Alexa Fluor 488 goat anti-mouse IgG secondary antibody at a dilution of 1/1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye.