Anti-Progesterone Receptor antibody - ChIP Grade (ab68195)


  • Product nameAnti-Progesterone Receptor antibody - ChIP Grade
    See all Progesterone Receptor primary antibodies
  • Description
    Rabbit polyclonal to Progesterone Receptor - ChIP Grade
  • Tested applicationsSuitable for: ChIP, ICC/IF, WB, IP, IHC-P, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to C-terminus of human Progesterone receptor.

  • Positive control
    • MCF-7 cell lysate and human breast cancer tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: DU145


Associated products


Our Abpromise guarantee covers the use of ab68195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
  • Application notesChIP: Use at an assay dependent dilution (PMID 19744932).
    IHC-P: Use at a concentration of 1 - 2 µg/ml.
    IP: Use at a concentration of 2 - 5 µg/ml.
    I-ELISA: Use at a concentration of 0.01 - 0.1 µg/ml.
    WB: Use at a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 99 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
      Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
      Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Post-translational
      Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
      Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
      Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
      Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
    • Cellular localizationNucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • NR3C3 antibody
      • Nuclear receptor subfamily 3 group C member 3 antibody
      • PGR antibody
      • PR antibody
      • PRA antibody
      • PRB antibody
      • PRGR_HUMAN antibody
      • Progesterone receptor antibody
      • Progestin receptor form A antibody
      • Progestin receptor form B antibody
      see all

    Anti-Progesterone Receptor antibody - ChIP Grade images

    • ICC/IF image of ab68195 stained DU145 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab68195 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-Progesterone Receptor antibody - ChIP Grade (ab68195) at 1/500 dilution + MCF-7 cell lysate

      Predicted band size : 99 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • ab68195 at a 1:200 dilution staining Progesterone Receptor in human breast cancer tissue.

    References for Anti-Progesterone Receptor antibody - ChIP Grade (ab68195)

    This product has been referenced in:
    • Chen L  et al. Cyclic AMP enhances progesterone action in human myometrial cells. Mol Cell Endocrinol 382:334-43 (2014). Human . Read more (PubMed: 24161591) »
    • Palijan A  et al. Ligand-dependent corepressor LCoR is an attenuator of progesterone-regulated gene expression. J Biol Chem 284:30275-87 (2009). ChIP ; Human . Read more (PubMed: 19744932) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (Mammary epithelial cells)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
    Permeabilization No
    Specification Mammary epithelial cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative Formaldehyde

    Mrs. Katie Elliott

    Verified customer

    Submitted Jul 15 2016