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Anti-Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764)

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Overview

Product name

Anti-Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade
See all Progesterone Receptor products (37) ...

Description

Mouse monoclonal [PR-AT 4.14] to Progesterone Receptor - ChIP Grade

Specificity

Detects progesterone receptor. This antibody does not cross-react with androgen receptor, estrogen receptor or glucocorticoid receptor.

Tested applications

IHC-Fr, IP, WB, IHC-P, ChIP, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Rabbit, Cow, Human, Rhesus monkey

Immunogen

Synthetic peptide: RPGEAAVTAAPASAS, corresponding to amino acids 433-447 of Human Progesterone Receptor(Peptide available as ab4907 .)

RPGEAAVTAAPASAS

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Rat uterus can be used for IHC.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS with 0.1% sodium azide

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

PR-AT 4.14

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Tumor biomarkers >> Receptors
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Progesterone
Neuroscience >> Endocrine system >> Gonadotrophic axis
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Progesterone
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

  • Immunofluorescence - Progesterone Receptor antibody [PR-AT 4.14] (ab2764)Immunofluorescence - Progesterone Receptor antibody [PR-AT 4.14] (ab2764) image (enlarge)

  • - Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764)- Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-Fr

     IHC-Fr: Use a concentrat...Read more →

    IHC-Fr: Use a concentration of 5 µg/ml. Immunohistochemical staining of PR in rat uterus with this antibody yields intense nuclear staining.

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

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    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent concentration. Predicted molecular weight: 99 kDa.By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from rat uterine homogenate. (PubMed: 16912069)

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    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 5 µg/ml.

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    ChIP

    Three stars (1 Abreview) ChIP: Use at an assay de...Read more →

    ChIP: Use at an assay dependent dilution. PubMed: 19553525

  • ShowHide1 Image

    ICC/IF

    Five stars (1 Abreview) ICC/IF: 1/500. Using T47...Read more →

    ICC/IF: 1/500. Using T47D cells.

Target

Function

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

Domain

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

Post-translational
modifications

Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.

Cellular localization

Nucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases and Nucleus. Cytoplasm. Mainly nuclear.

Target information above from: UniProt accessionP06401 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • NR3C3 antibody
  • NR3C3 antibody
  • Nuclear receptor subfamily 3 group C member 3 antibody
  • PGR antibody
  • PGR antibody
  • PR antibody
  • PR antibody
  • PRA antibody
  • PRB antibody
  • PRGR_HUMAN antibody
  • Progesterone receptor antibody
  • Progestin receptor form A antibody
  • Progestin receptor form B antibody
see all

Database links

see all

Anti-Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade images:

  Immunofluorescence - Progesterone Receptor antibody [PR-AT 4.14] (ab2764)

Immunofluorescence - Progesterone Receptor antibody [PR-AT 4.14] (ab2764)

T47D cells were treated with progesterone (10X-8M) for 2 hours, and then fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/1000 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-mouse Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted. Red indicates staining by ab2764, blue staining by DAPI.

Michael Mancini, Baylor College of Medicine

  - Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764)

- Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764)

ab2764 at a 1/400 dilution staining Progesterone Receptor in rat uterus tissue by Immunohistochemistry.

Tissue was fixed in formaldehyde and permeabilized using 0.3% Triton-X. 10% serum was used in the blocking step. The secondary used was a Goat anti-mouse conjugated to biotin, 1/500 dilution.

This antibody worked well in rat sections. However, in mouse sections, due to very poor homology between the immunogen used and the mouse nPR receptor, the antibody did not work despite taking steps to prevent mouse-on-mouse staining issues. So in short, this antibody is good for rat sections, where immunogen homology is better, but be careful about using it in mouse sections.

Image courtesy of Nicholas Bashour by Abreview.

References for Anti-Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764)

This product has been referenced in:

  • Liu JL  et al. Combined Analysis of MicroRNome and 3'-UTRome Reveals a Species-specific Regulation of Progesterone Receptor Expression in the Endometrium of Rhesus Monkey. J Biol Chem 287:13899-910 (2012). WB; Rhesus monkey.Read more (PubMed: 22378788) »
  • Yuan W  et al. Conditional activation of Pik3ca(H1047R) in a knock-in mouse model promotes mammary tumorigenesis and emergence of mutations. Oncogene : (2012). ICC/IF; Mouse.Read more (PubMed: 22370636) »

See all 12 publications for this product

Publishing research using ab2764? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"