Anti-Progesterone Receptor antibody [YR85] (ab32085)




Our Abpromise guarantee covers the use of ab32085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.
IP 1/150.
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionThe steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
    Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
    Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Post-translational
    Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G(2)/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1.
    Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294.
    Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294.
    Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation.
  • Cellular localizationNucleus. Cytoplasm. Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases; Mitochondrion outer membrane and Nucleus. Cytoplasm. Mainly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR3C3 antibody
    • Nuclear receptor subfamily 3 group C member 3 antibody
    • PGR antibody
    • PR antibody
    • PRA antibody
    • PRB antibody
    • PRGR_HUMAN antibody
    • Progesterone receptor antibody
    • Progestin receptor form A antibody
    • Progestin receptor form B antibody
    see all

Anti-Progesterone Receptor antibody [YR85] images

  • ICC/IF image of ab32085 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32085 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Progesterone Receptor antibody [YR85] (ab32085) at 1/10000 dilution + T47D cell lysate

    Predicted band size : 99 kDa
    Observed band size : 99,135 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of progesterone receptor expression in paraffin embedded human breast carcinoma, using 1/100 ab32085.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-Progesterone Receptor antibody [YR85] (ab32085)

This product has been referenced in:
  • Yin K Positive correlation between expression level of mitochondrial serine hydroxymethyltransferase and breast cancer grade. Onco Targets Ther 8:1069-74 (2015). IHC-P ; Human . Read more (PubMed: 25999742) »
  • Argenta PA  et al. Predicting response to the anti-estrogen fulvestrant in recurrent ovarian cancer. Gynecol Oncol N/A:N/A (2013). ICC/IF ; Human . Read more (PubMed: 23911795) »

See all 9 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (Mammary epithelial cells)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate buffer
Permeabilization No
Specification Mammary epithelial cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde

Mrs. Katie Elliott

Verified customer

Submitted Jul 15 2016

Es tut mir leid, dass der Antikörper nicht so funktioniert hat wie auf dem Datenblatt angegeben. Ich habe unsere Finanzabteilung beauftragt, Gutschriften für Sie auszustellen. Sie können die Gutschriften auf folgende Weise verwenden:

(1) Gegen...

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Wir testen unsere Chargen immer, bevor wir unser Datenblatt aktualisieren, und uns ist bei diesem Antikörper aufgefallen, dass die Spezies- Reaktivität in Maus und Ratte eben nicht konstant, bzw gar nicht mehr gegeben war, so dass wir Maus und Ratte al...

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Vielen Dank für Ihre Email.

Wir haben das Datenblatt im März 2011 geändert, allerdings habe ich keine Hinweise darauf gefunden, dass wir eine Kampagne gestartet haben. Ich habe dies jetzt nachgeholt.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human breast cancer xenograft (MCF7))
Specification human breast cancer xenograft (MCF7)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6.0
Permeabilization No
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (breast cancer)
Specification breast cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate - 20 minutes 100oC
Permeabilization No

Abcam user community

Verified customer

Submitted Feb 09 2009

Application ChIP
Sample Human Cell lysate - whole cell (Uterus)
Specification Uterus
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Semiquantitative PCR
Positive control RNA Polymerase II
Negative control Rabbit IgG

Abcam user community

Verified customer

Submitted Oct 17 2008

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab32085 in immunohistochemistry. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and...

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