Overview

  • Product name
  • Description
    Rabbit polyclonal to Prohibitin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Cat, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 23-272 of Human Prohibitin (Uniprot ID: P35232).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and NIH 3T3 whole cell lysates; HeLa cells; Human OVCAR3 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-Prohibitin antibody (ab154589) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size: 30 kDa



    12% SDS PAGE
  • All lanes : Anti-Prohibitin antibody (ab154589) at 1/3000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Confocal immunofluorescence analysis of methanol-fixed HeLa cells, labeling Prohibitin with ab154589 at 1/500 dilution (Green). Alpha-tubulin filaments are labeled in red.
  • Immunohistochemical analysis of paraffin-embedded Human OVCAR3 xenograft tissue labeling Prohibitin with ab154589 at 1/500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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