Overview

  • Product name
  • Description
    Mouse monoclonal to Prohibitin
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-273 of Human Prohibitin

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab55618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 30 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • Tissue specificity
    Widely expressed in different tissues.
  • Sequence similarities
    Belongs to the prohibitin family.
  • Developmental stage
    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

Images

  • ICC/IF image of rat primary mixed glial culture stained with ab55618. The cells on cover slip were incubated in 10% normal donkey serum in 0.1% PBS- and 0.3x triton1000 for 1h to permeabilise the cells and block non-specific protein-protein interactions. The sections were then incubated with the antibody (ab55618, 1µg/ml) overnight at +4°C. The secondary antibody was Alexa Fluor®568 donkey anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Prohibitin antibody (ab55618) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • Prohibitin antibody (ab55618) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human tonsil tissue.


  • Predicted band size : 30 kDa
    Prohibitin antibody (ab55618) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HepG2 cells stained with ab55618 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55618, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Ichikawa Y  et al. Modulation of caveolins, integrins and plasma membrane repair proteins in anthracycline-induced heart failure in rabbits. PLoS One 12:e0177660 (2017). WB . Read more (PubMed: 28498861) »
  • Kamal AH  et al. Investigation of adipocyte proteome during the differentiation of brown preadipocytes. J Proteomics 94:327-36 (2013). Read more (PubMed: 24129212) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cell (Mature spermatid/Adult testes)
Permeabilization
Yes - 0.4% PBS Triton
Specification
Mature spermatid/Adult testes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 11 2015

Application
Western blot
Sample
Mouse Tissue lysate - other (Liver Mitochondrial fraction)
Loading amount
20 µg
Specification
Liver Mitochondrial fraction
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Mixed Glial culture)
Specification
Mixed Glial culture
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1x PBS plus 0.3xTriton x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 21 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Loading amount
2 µg
Specification
HL60
Gel Running Conditions
Reduced Denaturing (17%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 16 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Formaldehyde
Permeabilization
Yes - Triton 0.6%
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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