Overview

  • Product name
  • Description
    Rabbit polyclonal to Prohibitin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to near C terminus of human Prohibitin protein.

  • Positive control
    • Human heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab70672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
  • Application notes
    IHC-P: 1/100 for 30 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

    WB: Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
    • Tissue specificity
      Widely expressed in different tissues.
    • Sequence similarities
      Belongs to the prohibitin family.
    • Developmental stage
      Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
    • Cellular localization
      Mitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Epididymis luminal protein 215 antibody
      • Epididymis secretory sperm binding protein Li 54e antibody
      • HEL 215 antibody
      • HEL S 54e antibody
      • PHB antibody
      • PHB_HUMAN antibody
      • PHB1 antibody
      • Prohibitin antibody
      see all

    Images

    • ab70672, at 1/100 dilution, staining Prohibitin in formalin fixed, paraffin embedded human heart tissue by immunohistochemistry.

    References

    This product has been referenced in:
    • Zhu X  et al. Long-circulating siRNA nanoparticles for validating Prohibitin1-targeted non-small cell lung cancer treatment. Proc Natl Acad Sci U S A 112:7779-84 (2015). Read more (PubMed: 26056316) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you very much for your patience.

    I have hear back now from the laboratories in regards to the potential crossreaction of the prohibitin antibodies with T. brucei. Unfortunately none of these antibodies seems to be likely to crossreact w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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