Anti-Prohibitin antibody [II-14-10] (ab1836)

Overview

  • Product name
    Anti-Prohibitin antibody [II-14-10]
    See all Prohibitin primary antibodies
  • Description
    Mouse monoclonal [II-14-10] to Prohibitin
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    BALB/C mice were injected with purified recombinant rat prohibitin protein.

  • Positive control
    • Human Heart
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab75771 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab1836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/100. ABC method.
IP Use at an assay dependent concentration.
WB 1/100. (see Abreview).
Flow Cyt Use 1µg for 106 cells.

Target

  • Function
    Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
  • Tissue specificity
    Widely expressed in different tissues.
  • Sequence similarities
    Belongs to the prohibitin family.
  • Developmental stage
    Levels of expression in fibroblasts decrease heterogeneously during cellular aging.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis luminal protein 215 antibody
    • Epididymis secretory sperm binding protein Li 54e antibody
    • HEL 215 antibody
    • HEL S 54e antibody
    • PHB antibody
    • PHB_HUMAN antibody
    • PHB1 antibody
    • Prohibitin antibody
    see all

Images

  • Formalin fixed paraffin embedded human heart stained with Prohibitin antibody ab1836.
  • ab1836 at a 1/100 dilution staining mouse Cardiac muscle tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the antibody was incubated with the tissue for 12 hours. Bound antibody was detected with a HRP conjuaged anti-mouse antibody.

    The picture shown is a transverse section of mouse ventricular wall; from TEM studies the distribution of mitochondria among the myofibrils is well-established, and so in this 2-um-thick section there exists both minimal superposition as well as a staining pattern which is consistent with what is expected for cardiac mitochondrial patterns (arrows in the inset indicate groups of prohibitin-positive mitochondria).

    This image is courtesy of an Abreview submitted by Mike Forbes on 29 March 2006.

    See Abreview

  • ICC/IF image of ab1836 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1836, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti- IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

  • ab1836 staining Prohibitin in normal human skin tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 10% serum for 30 minutes at 21°C; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/200 in TBS + 1% BSA) at 21°C for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody.

    See Abreview

  • Overlay histogram showing HeLA cells stained with ab1836 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1836, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocellular carcinoma tissue, staining Prohibitin with ab1836. Staining was detected using DAB.

References

This product has been referenced in:
  • Cao Y  et al. Prohibitin overexpression predicts poor prognosis and promotes cell proliferation and invasion through ERK pathway activation in gallbladder cancer. J Exp Clin Cancer Res 35:68 (2016). Read more (PubMed: 27084680) »
  • Zhu X  et al. Long-circulating siRNA nanoparticles for validating Prohibitin1-targeted non-small cell lung cancer treatment. Proc Natl Acad Sci U S A 112:7779-84 (2015). Read more (PubMed: 26056316) »

See all 17 Publications for this product

Customer reviews and Q&As



Unfortunately, the homology between the mouse protein and the target protein is too low with 75%, so that a cross-reactivity is less likely in this case. This means, that on this occasion we cannot give your customer our AbTrial discount.
Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Standard
Permeabilization
No
Blocking step
S Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Dec 11 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Normal Skin)
Specification
Normal Skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat, pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Placenta)
Loading amount
20 µg
Specification
Placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Victoria Roberts

Verified customer

Submitted Jan 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cardiac muscle)
Specification
Cardiac muscle
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Username

Mike Forbes

Verified customer

Submitted Mar 29 2006

Thank you for your enquiry and your patience in awaiting a response. This product does contain both BSA and sodium azide. I have updated the on line data sheet to reflect this additional information. I am currently in contact with originator of this pr...

Read More

Thank you for your recent enquiry regarding the concentration of ab1836. The current lot is 100 ug/ml. Please do not hesitate to contact us again if you need further information,

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. Ab1836 has not yet been tested for application in confocal microscopy so I am unable to give you a description as to how to use it ...

Read More

The ABC method is mainly used in IHC and involves an avidin-biotin complex. We do not have recommended dilutions for western blot as is depends on the assay used. Instead we recommend using Abcam's general protocols and adjusting your results accordingly.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up