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BALB/C mice were injected with purified recombinant rat prohibitin protein.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab75771 as a replacement.
Our Abpromise guarantee covers the use of ab1836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.|
|IHC-Fr||1/50 - 1/100. ABC method.|
|IP||Use at an assay dependent concentration.|
|WB||1/100. (see Abreview).|
|Flow Cyt||Use 1µg for 106 cells.|
ab1836 at a 1/100 dilution staining mouse Cardiac muscle tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the antibody was incubated with the tissue for 12 hours. Bound antibody was detected with a HRP conjuaged anti-mouse antibody.
The picture shown is a transverse section of mouse ventricular wall; from TEM studies the distribution of mitochondria among the myofibrils is well-established, and so in this 2-um-thick section there exists both minimal superposition as well as a staining pattern which is consistent with what is expected for cardiac mitochondrial patterns (arrows in the inset indicate groups of prohibitin-positive mitochondria).
This image is courtesy of an Abreview submitted by Mike Forbes on 29 March 2006.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"