The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
Does not displace peptide in range 0.1-1000 µg/ml, with 1 µg/well of pep-BSA coated.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceProhormone Convertase 1 (PC1) is a serine proteinase responsible for the proteolytic processing of many precursor proteins within the regulated secretory pathway. The activity of PC1 is potentially regulated by two endogenous inhibitors, the PC1 propeptide and proSAAS.
Prohormone Convertase 2 (PC2) is a subtilisin-like proprotein convertase, which is expressed extensively in mammalian neural and endocrine cells. PC2 plays a major role in the proteolytic processing of both neuropeptide and peptide hormone precursors. PC2 is expressed at high levels in the islets of Langerhans, where it participates in the processing of proinsulin to insulin.
Prohormone convertase 3 (PC3) is a neuroendocrine-specific member of the subtilisin-kexin family, involved in the intracellular processing and maturation of prohormones and proneuropeptides.
Cellular localizationPC1 and PC2: Cytoplasmic vesicle, secretory vesicle. Note=Localized in the secretion granules.
PC3: Golgi apparatus, trans-Golgi network. Note=Seems to be localized intracellularly to the trans Golgi network. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). Second cleavage in the propeptide occur in the trans Golgi network (TGN), which is followed by the release of the propeptide bound to furin and the activation of furin.