Overview

  • Product nameAnti-Proinsulin antibody [3A1]
    See all Proinsulin primary antibodies
  • Description
    Mouse monoclonal [3A1] to Proinsulin
  • SpecificityKd for this antibody is >1.1 x 10-8M This antibody is specific for proinsulin. It does not recognise insulin or C-peptide.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Fr, RIAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Cow, Pig
  • Immunogen

    Full length native protein (purified) (Human).

  • EpitopeThis antibody reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab8301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

Target

  • RelevanceA precursor of insulin it is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • INS antibody
    • insulin antibody
    • IRDN antibody
    • Proinsulin precursor antibody

Anti-Proinsulin antibody [3A1] images

  • ab8301 at a 1/500 dilution staining human pancreas tissue sections by immunohistochemistry (frozen). The antibody was incubated with the tissue for 1 hour and then detected using an HRP conjugated system.

    This image is courtesy of an Abreview submitted by an anonymous researcher on 12 December 2005.

    See Abreview

References for Anti-Proinsulin antibody [3A1] (ab8301)

This product has been referenced in:
  • Cao M  et al. PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biol 11:e1001541 (2013). Read more (PubMed: 23630453) »
  • Colombo C  et al. Seven mutations in the human insulin gene linked to permanent neonatal/infancy-onset diabetes mellitus. J Clin Invest : (2008). ICC/IF ; Human . Read more (PubMed: 18451997) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - citrate buffer
Specification Brain
Blocking step 10% Methanol+3% H2O2 in PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
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Verified customer

Submitted Sep 23 2015

Vielen Dank für Ihre Anfrage. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 auch nicht mit Mausproben im ELISA funktioniert. Wenn ich mich richtig erinnere, hatten wir am Telefon jedoch auch besprochen, dass ab8301 bisher nicht in der Mau...

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Vielen Dank für diese Rückmeldung. Es tut mir leid, dass der Proinsulin- Antikörper ab8301 nicht optimal für Ihre Zwecke geeignet ist. Leider ist eine nur sehr geringe Verdünnung keiner der Gründe, bei dem es mir erlaubt ist, einen kostenlosen Ersa...

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Thank you for your inquiry. Ab8301 reacts with the site of connection of C-peptide and C-end of B-chain of proinsulin molecule. According to our info, ab8301 has been tested only with unprocessed proinsulin. I hope that this information is helpfu...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (frozen and FFPE pancreas)
Specification frozen and FFPE pancreas
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1
Username

Abcam user community

Verified customer

Submitted Dec 12 2005

Clone 3A1 (ab8301) reacts with the site of connection of C-peptide and C-end of B-chain of the proinsulin molecule. Clones 1D6 (ab8299) and 1D3 (ab8300) react with different epitopes on the human proinsulin molecule, both of each are generated by the ...

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These are some of our newest products and all the information we have on each of them is featured on the datasheets. As yet, there are no published references. Pricing information in GBP, EUR and USD can be found by clicking on the "prices and buying" ...

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The antibody has not yet been tested on paraffin embedded sections, although it has been shown to be suitable for use in IHC with frozen sections. It has not been tested on mouse although we note that it does not cross-react with bovine or porcine proi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"