Overview

  • Product nameAnti-Proinsulin antibody [C-PEP-01]
    See all Proinsulin primary antibodies
  • Description
    Mouse monoclonal [C-PEP-01] to Proinsulin
  • Tested applicationsSuitable for: ELISA, IHC-P, RIA, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    C-peptide (Human) conjugated to BSA.

  • General notes


    The antibody can be used for RIA and ELISA estimation of human proinsulin C-peptide or human proinsulin in body fluids.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites using precipitation methods.
  • Primary antibody notesThe antibody can be used for RIA and ELISA estimation of human proinsulin C-peptide or human proinsulin in body fluids.
  • ClonalityMonoclonal
  • Clone numberC-PEP-01
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab7761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P 1/200 - 1/500.
RIA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

Target

  • RelevanceA precursor of insulin it is converted to insulin by removal of the connecting C peptide, leaving the two (A and B) chains. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • INS antibody
    • insulin antibody
    • IRDN antibody
    • Proinsulin precursor antibody

Anti-Proinsulin antibody [C-PEP-01] images

  • Ab7761 staining human normal pancreas tissue. Staining is localised to extracellular space.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Proinsulin antibody [C-PEP-01] (ab7761)

This product has been referenced in:
  • Hilgert I  et al. A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA. Hybridoma 10:379-86 (1991). Read more (PubMed: 1916848) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This antibody is very specific and does not cross-react with active insulin. Please do not hesitate to contact us if you have additional questions.

Thank you for your enquiry regarding ab12762(25µg), ab12764(25µg), ab12769(25µg), ab11022(1ml), ab10988(200µl) and ab7761(100µg). These products are all priced at $295 US dollars. In the future you can view pricing,availability and detailed information...

Read More

This antibody has only been tested on human pro-insulin. You will have to compare the sequences of the rat and human to determine whether you believe there may be some cross-reactivity.

This antibody works well in ELISA, but we have no data yet for its use on rat and hamster tissues.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"