Anti-Prokineticin 1 antibody (Biotin) (ab84014)

Overview

  • Product name
    Anti-Prokineticin 1 antibody (Biotin)
    See all Prokineticin 1 primary antibodies
  • Description
    Goat polyclonal to Prokineticin 1 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant human Prokineticin 1.

Properties

  • Form
    Lyophilised:Centrifuge vial prior to opening. Reconstitute in 50 µl of sterile PBS containing 0.1 % BSA to give a concentration of 1.0 mg/ml.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84014 is sterile filtered. ab84014 was purified by affinity chromatography and then biotinylated.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Sandwich ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Prokineticin 1.
    sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human Prokineticin 1.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant human Prokineticin 1 is 1.5 - 3.0 ng/lane, under either reducing or non reducing conditions. Predicted molecular weight: 12 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
    • Tissue specificity
      Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.
    • Sequence similarities
      Belongs to the AVIT (prokineticin) family.
    • Developmental stage
      In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Black mamba toxin related protein antibody
      • EG VEGF antibody
      • EG-VEGF antibody
      • EGVEGF antibody
      • Endocrine-gland-derived vascular endothelial growth factor antibody
      • Mambakine antibody
      • PK1 antibody
      • PRK1 antibody
      • PROK1 antibody
      • PROK1_HUMAN antibody
      • Prokineticin-1 antibody
      see all

    References

    ab84014 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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