Recombinant
RabMAb

Anti-Prokineticin 1 antibody [EPR9699] (ab150375)

Overview

  • Product name
    Anti-Prokineticin 1 antibody [EPR9699]
    See all Prokineticin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR9699] to Prokineticin 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Prokineticin 1 aa 50-150 (C terminal). The exact sequence is proprietary.
    (Peptide available as ab206122)

  • Positive control
    • Raw 264.7, NIH/3T3, and ECV-304 cell lysates; NIH/3T3 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR9699
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis.
    • Tissue specificity
      Localizes to glandular epithelium, stroma and vascular epithelial cells of first trimester decidua (at protein level). Up-regulated in first trimester decidua when compared with non-pregnant endometrium. Expressed in the steroidogenic glands, ovary, testis, adrenal and placenta.
    • Sequence similarities
      Belongs to the AVIT (prokineticin) family.
    • Developmental stage
      In adult testis, is strongly expressed only in Leydig cells. In testicular tumors, expressed specifically in Leydig cell tumors (at protein level). Expressed from 14 weeks until birth in fetal testis.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Black mamba toxin related protein antibody
      • EG VEGF antibody
      • EG-VEGF antibody
      • EGVEGF antibody
      • Endocrine-gland-derived vascular endothelial growth factor antibody
      • Mambakine antibody
      • PK1 antibody
      • PRK1 antibody
      • PROK1 antibody
      • PROK1_HUMAN antibody
      • Prokineticin-1 antibody
      see all

    Images

    • All lanes : Anti-Prokineticin 1 antibody [EPR9699] (ab150375) at 1/1000 dilution

      Lane 1 : Raw 264.7 cell lysate.
      Lane 2 : NIH/3T3 cell lysate.
      Lane 3 : ECV-304 cell lysate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 12 kDa

    • Flow cytometric analysis of permeabilized NIH/3T3 cells labelling Prokineticin 1 with ab150375 at 1/10 dilution (red) or a Rabbit IgG (negative, green).

    References

    ab150375 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150375.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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