Anti-Prokineticin 2 antibody (ab87360)

Overview

  • Product nameAnti-Prokineticin 2 antibody
    See all Prokineticin 2 primary antibodies
  • Description
    Rabbit polyclonal to Prokineticin 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Prokineticin 2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Testis; Human Small Intestine; Mouse Testis; Rat Testis.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab87360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 14 kDa).

Target

  • FunctionMay function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle.
  • Tissue specificityExpressed in the testis and, at low levels, in the small intestine.
  • Involvement in diseaseDefects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4) [MIM:610628]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis.
  • Sequence similaritiesBelongs to the AVIT (prokineticin) family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • BV8 antibody
    • Bv8 homolog antibody
    • MIT1 antibody
    • PK2 antibody
    • PROK2 antibody
    • PROK2_HUMAN antibody
    • Prokineticin-2 antibody
    • Protein Bv8 homolog antibody
    see all

Anti-Prokineticin 2 antibody images

  • All lanes : Anti-Prokineticin 2 antibody (ab87360) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : Testis (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Prokineticin 2 antibody (ab87360)

ab87360 has not yet been referenced specifically in any publications.

Product Wall


L'anticorps anti-Prokineticin 2 ab87360 a été caractérisé en Western Blot selon le protocole suivant:

Conditions dénaturantes et réductrices
10µg de lysat par puit
Saturation de la m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"