Overview

  • Product name
  • Description
    Goat polyclonal to Prol1
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide:

    QFPVRKYLEDPRY

    with a Cysteine residue linker, corresponding to internal sequence amino acids 30-42 of Mouse Prol1 (NP_032670.2).

Properties

Applications

Our Abpromise guarantee covers the use of ab119999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
ICC/IF Use a concentration of 3 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC50 values of 29 µM, 33 µM and 33 µM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC50 of 65 µM. Has a potent analgesic effect when administered to rats by intravenous injection.
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Basic proline rich lacrimal protein antibody
    • Basic proline rich protein antibody
    • BPLP antibody
    • PRL1 antibody
    • Proline rich 1 antibody
    • Proline rich lacrimal 1 antibody
    • Proline rich protein 1 antibody
    see all

Images

  • ab119999 (2.9ug/ml) staining of cells in the submandibular salivery gland (SMG), but not in the sublingual salivery gland (SLG) in mouse.

References

ab119999 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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