The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. Preliminary experiments gave an approx 45kDa band in Mouse Eye lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa according to NP_032670.2. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Use a concentration of 3 µg/ml.
Use at an assay dependent concentration.
Opiorphin is an endogenous inhibitor of neprilysin and aminopeptidase N. Inhibits the breakdown of substance P, Mca-BK2 and Met-enkephalin by neprilysin in vitro with IC50 values of 29 µM, 33 µM and 33 µM respectively. Inhibits the breakdown of Ala-pNA by aminopeptidase N in vitro with an IC50 of 65 µM. Has a potent analgesic effect when administered to rats by intravenous injection.