Prolactin overexpression 293T lysate (whole cell) (ab94057)

Overview

  • Product nameProlactin overexpression 293T lysate (whole cell)
  • Description
    Prolactin overexpression lysate
  • General notesab94057 is a 293T cell transfected lysate in which Human Prolactin has been transiently over-expressed using a pCMV-Prolactin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundVarious hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Prolactin overexpression 293T lysate (whole cell) images

  • ab94057 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-Prolactin antibody (ab57741) at 1/500 dilution

    Lane 1 : Prolactin overexpression 293T lysate (whole cell) (ab94057)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for Prolactin overexpression 293T lysate (whole cell) (ab94057)

ab94057 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"