The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 69 kDa).
This is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Expressed in breast, placenta, kidney, liver and pancreas.
Belongs to the type I cytokine receptor family. Type 1 subfamily. Contains 2 fibronectin type-III domains.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Western blot - Prolactin Receptor antibody (ab87992)
All lanes : Anti-Prolactin Receptor antibody (ab87992) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Human kidney tissue lysate - total protein (ab30203) Lane 3 : Human breast tissue lysate - total protein (ab30090) Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution