The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: Ready to use. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThis is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
Tissue specificityExpressed in breast, placenta, kidney, liver and pancreas.
Sequence similaritiesBelongs to the type I cytokine receptor family. Type 1 subfamily. Contains 2 fibronectin type-III domains.
DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.