Anti-Prolactin Receptor antibody [T6] (ab2773)

Overview

  • Product nameAnti-Prolactin Receptor antibody [T6]
    See all Prolactin Receptor primary antibodies
  • Description
    Mouse monoclonal [T6] to Prolactin Receptor
  • SpecificityDetects prolactin (PRL) receptor in rat tissues. This antibody does not cross-react with Growth Hormone (GH) receptor. By Western blot, this antibody detects an ~42 kDa protein representing PRL receptor in NB2 cell lysate. Immunohistochemical staining of PRL receptor in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining. This antibody has also been used to inhibit the binding of prolactin to prolactin receptor in vitro.
  • Tested applicationsSuitable for: ICC, IHC-Fr, WB, IP, Flow Cyt, Inhibition Assay, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Other Immunogen Type corresponding to Rat Prolactin Receptor. Purified rat liver PRL receptor.

  • Positive control
    • WB and ICC: NB2 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesProlactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
  • ClonalityMonoclonal
  • Clone numberT6
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
ICC/IF 1/200.

Target

  • FunctionThis is a receptor for the anterior pituitary hormone prolactin (PRL). Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
  • Tissue specificityExpressed in breast, placenta, kidney, liver and pancreas.
  • Sequence similaritiesBelongs to the type I cytokine receptor family. Type 1 subfamily.
    Contains 2 fibronectin type-III domains.
  • DomainThe WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • Cellular localizationSecreted and Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI987712 antibody
    • CLONE SPM213 antibody
    • CPRLP antibody
    • Delta 4-delta 7/11 truncated prolactin receptor antibody
    • Delta 4-SF1b truncated prolactin receptor antibody
    • HPRL antibody
    • hPRL receptor antibody
    • hPRLrI antibody
    • Lactogen receptor antibody
    • MFAB antibody
    • MGC105486 antibody
    • OPR antibody
    • OTTHUMP00000115998 antibody
    • Pr-1 antibody
    • Pr-3 antibody
    • PRL R antibody
    • PRL-R antibody
    • PRLR antibody
    • Prlr-rs1 antibody
    • PRLR_HUMAN antibody
    • Prolactin receptor a antibody
    • Prolactin receptor antibody
    • Prolactin receptor delta 7/11 antibody
    • RATPRLR antibody
    • Secreted prolactin binding protein antibody
    • Truncated testis-specific box 1-C prolactin receptor antibody
    • wu:fj65c07 antibody
    see all

Anti-Prolactin Receptor antibody [T6] images

  • Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in C6 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in H-4-II-E cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunocytochemistry/Immunofluorescence analysis of Prolactin Receptor in SW480 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were incbated without (control) or with ab2773 (1:200) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Prolactin Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized rat pituitary gland tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2773 (1:50) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

References for Anti-Prolactin Receptor antibody [T6] (ab2773)

This product has been referenced in:
  • Dardenne M  et al. Prolactin receptor expression in human hematopoietic tissues analyzed by flow cytofluorometry. Endocrinology 134:2108-14 (1994). Read more (PubMed: 8156910) »
  • Rui H  et al. JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization. Endocrinology 135:1299-306 (1994). Read more (PubMed: 7925093) »

See all 6 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (liver, mammary gland)
Specification liver, mammary gland
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6.0
Permeabilization No
Blocking step protein block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: rt°C
Username

Ms. Francine Deslippe

Verified customer

Submitted Apr 10 2013

Thank you for contacting us.
I am sorry to hear about the problems you have encountered.

The bands you are seeing at 25 kDa could be the light chain of endogenous IgG.

As we discussed over the phone, please try the following su...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Forebrain)
Loading amount 40 µg
Specification Forebrain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Jan 08 2007

Here is a reference that uses our monoclonal antibodies with IHC procedures: Rl Sorenson and LE Stout. Prolactin Receptors and JAK2 in Islets of Langerhans: An Immunohistochemical Analysis. Endocrinology 136:9 pp 4092-4098. 1995 Also used in...

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Ab2773 has been shown to be specific for the short form around 42 kDa. The other Prolactin Receptor antibody that we currently have, ab2772, detects both the short and long forms. So unfortunately we don't have what your customer is looking for.

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"