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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab86094 was affinity purified using an epitope specific to Proliferation Associated Nucleolar Antigen immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
Application notes Is unsuitable for IP.
Relevance Proliferation-associated nucleolar antigen is expressed in mid G1 phase with peak level during the S phase and a rapid degradation during late mitosis. Its expression in breast carcinoma is correlated with patient prognosis.
Cellular localization Nuclear
NUCLEOLAR ANTIGEN p120 antibody
Anti-Proliferation Associated Nucleolar Antigen antibody images
Western blot - Proliferation Associated Nucleolar Antigen antibody (ab86094)
All lanes : Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg/ml Lane 2 : HeLa whole cell lysate at 15 µg/ml Lane 3 : HeLa whole cell lysate at 5 µg/ml Predicted band size : 95 kDa Observed band size : 117 kDa ( why is the actual band size different from the predicted?) Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands. Exposure time : 60 seconds
References for Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"