Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094)

Overview

  • Product nameAnti-Proliferation Associated Nucleolar Antigen antibody
  • Description
    Rabbit polyclonal to Proliferation Associated Nucleolar Antigen
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 762-812 of Human Proliferation Associated Nucleolar Antigen (AAH00656.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab86094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • RelevanceProliferation-associated nucleolar antigen is expressed in mid G1 phase with peak level during the S phase and a rapid degradation during late mitosis. Its expression in breast carcinoma is correlated with patient prognosis.
    • Cellular localizationNuclear
    • Database links
    • Alternative names
      • NOL1 antibody
      • NUCLEOLAR ANTIGEN p120 antibody

    Anti-Proliferation Associated Nucleolar Antigen antibody images

    • All lanes : Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg/ml
      Lane 2 : HeLa whole cell lysate at 15 µg/ml
      Lane 3 : HeLa whole cell lysate at 5 µg/ml


      Predicted band size : 95 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 60 seconds

    References for Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094)

    ab86094 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"