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Anti-Proliferation Associated Nucleolar Antigen antibody
Rabbit polyclonal to Proliferation Associated Nucleolar Antigen
Predicted to work with:
Synthetic peptide, corresponding to a region between amino acids 762-812 of Human Proliferation Associated Nucleolar Antigen (AAH00656.1)
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab86094 was affinity purified using an epitope specific to Proliferation Associated Nucleolar Antigen immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
Is unsuitable for IP.
Proliferation-associated nucleolar antigen is expressed in mid G1 phase with peak level during the S phase and a rapid degradation during late mitosis. Its expression in breast carcinoma is correlated with patient prognosis.
NUCLEOLAR ANTIGEN p120 antibody
Western blot - Proliferation Associated Nucleolar Antigen antibody (ab86094)
All lanes :
Anti-Proliferation Associated Nucleolar Antigen antibody (ab86094) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg/ml
Lane 2 :
HeLa whole cell lysate at 15 µg/ml
Lane 3 :
HeLa whole cell lysate at 5 µg/ml
Predicted band size :
Observed band size :
117 kDa (
why is the actual band size different from the predicted?
Additional bands at :
71 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"