Anti-Prolyl Endopeptidase antibody (ab110857)

Overview

  • Product nameAnti-Prolyl Endopeptidase antibody
    See all Prolyl Endopeptidase primary antibodies
  • Description
    Goat polyclonal to Prolyl Endopeptidase
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide:

    C-QYIVGRSRKQSNP

    , corresponding to internal sequence amino acids 657-669 of Human Prolyl Endopeptidase.

  • Positive control
    • Human kidney and tonsil tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99.48% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab110857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Anti-Prolyl Endopeptidase antibody images

  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab110857 at 3.75 µg/ml staining Prolyl Endopeptidase in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.

References for Anti-Prolyl Endopeptidase antibody (ab110857)

ab110857 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako Flex peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative 10% buffered formalin
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Submitted Oct 23 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Sample Rat Tissue sections (kidney)
Specification kidney
Permeabilization Yes - TritonX-100
Fixative Formaldehyde
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Submitted Mar 19 2014

Thank you for contacting us. Please find a protocol attached for performing heat-mediated antigen retrieval in free floating sections (PMID: 10634500). Additionally, the following website provides a protocol for performing heat-mediated antigen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"