Anti-Prolyl Endopeptidase antibody (ab58988)
Key features and details
- Rabbit polyclonal to Prolyl Endopeptidase
- Suitable for: WB
- Reacts with: Dog
- Isotype: IgG
Overview
-
Product name
Anti-Prolyl Endopeptidase antibody
See all Prolyl Endopeptidase primary antibodies -
Description
Rabbit polyclonal to Prolyl Endopeptidase -
Host species
Rabbit -
Specificity
ab58988 does not recognize other Prolyl Endopeptidase family members. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Dog -
Immunogen
Synthetic peptide based on the amino terminal end of human Prolyl Endopeptidase. (Peptide available as ab63973.) Immunizing peptide available on request, please contact a customer service representative to ask for availability.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Notes |
---|
A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates.
EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
-
Function
Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. -
Sequence similarities
Belongs to the peptidase S9A family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Alternative names
- dJ355L5.1 (prolyl endopeptidase) antibody
- HGNC:9358 antibody
- MGC16060 antibody
see all
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (18)
ab58988 has been referenced in 18 publications.
- Minucci S & Venditti M New Insight on the In Vitro Effects of Melatonin in Preserving Human Sperm Quality. Int J Mol Sci 23:N/A (2022). PubMed: 35563519
- Zhang J et al. Prolyl endopeptidase disruption reduces hepatic inflammation and oxidative stress in methionine-choline-deficient diet-induced steatohepatitis. Life Sci 270:119131 (2021). PubMed: 33516698
- Venditti M et al. Preliminary Investigation on the Ameliorative Role Exerted by D-Aspartic Acid in Counteracting Ethane Dimethane Sulfonate (EDS) Toxicity in the Rat Testis. Animals (Basel) 11:N/A (2021). PubMed: 33435542
- Venditti M et al. Preliminary Investigation on the Involvement of Cytoskeleton-Related Proteins, DAAM1 and PREP, in Human Testicular Disorders. Int J Mol Sci 22:N/A (2021). PubMed: 34360857
- Jiang DX et al. Prolyl endopeptidase gene disruption attenuates high fat diet-induced nonalcoholic fatty liver disease in mice by improving hepatic steatosis and inflammation. Ann Transl Med 8:218 (2020). PubMed: 32309365