The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500. Detects a band of approximately 112-115 kDa (predicted molecular weight: 92 kDa).
Tissue specificityPresent in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
Sequence similaritiesBelongs to the prominin family.
Cellular localizationApical cell membrane. Basolateral cell membrane. Cell projection > microvillus membrane. Cell projection > cilium membrane. Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells.