Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab74997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 112-115 kDa (predicted molecular weight: 92 kDa).
ELISA 1/2000.

Target

  • Tissue specificityPresent in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
  • Sequence similaritiesBelongs to the prominin family.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationApical cell membrane. Basolateral cell membrane. Cell projection > microvillus membrane. Cell projection > cilium membrane. Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • hPROML2 antibody
    • MGC138714 antibody
    • OTTHUMP00000160939 antibody
    • OTTHUMP00000207357 antibody
    • PROM 2 antibody
    • PROM-2 antibody
    • PROM2 antibody
    • PROM2_HUMAN antibody
    • Prominin like protein 2 antibody
    • Prominin related protein antibody
    • Prominin-2 antibody
    • Prominin-like protein 2 antibody
    • PROML2 antibody
    see all

References for Anti-Prominin 2 antibody (ab74997)

ab74997 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Rat Cell (Epithelium)
Specification Epithelium
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. Sam Dowland

Verified customer

Submitted Sep 25 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"