Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.
For Microscopy analysis: PI can be viewed using rhodamine(red) filter (?= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).
Flow Cytometry analysis: PI staining can be monitored in FL2 (DNA content) or FL3 (viability) channel.
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|Propidium Iodide (1mg/ml)||ab14083||1 x 1ml|
Our Abpromise guarantee covers the use of ab14083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
Vero-DogSLAM (VDS) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400).
Wang et al. used ab14083 to investigate an optimized platform for maintaining proliferation of giant panda mesenchymal stem cells (MSCs). MSCs were fixed in 70% alcohol and 30% PBS at 4°C for 1 hour. Cells are then incubated in the dark with PBS, 20µg/ml propidium iodide (ab14083) and 1% RNaseA for 30 minutes at 37°C. Cell samples are resuspended in PBS and analyzed using FACS Calibur flow cytometry.
Flow cytometry analysis shows the cells are at different cell cycle stages in different concentrations (0ng/ml and 5ng/ml respectively) of basic fibroblast growth factor (bFGF).