Proprotein Convertase 2 antibody - Homo-B domain (ab28572)

Product Name 

Proprotein Convertase 2 antibody - Homo-B domain

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Proprotein Convertase 2 - Homo-B domain

Immunogen 

Synthetic peptide corresponding to the carboxy end of the Homo B domain of human PC2.

(Peptide available as ab41408.)

Reacts with 
(species key)

Hu

Specificity 

ab28572 recognises the Homo B domain of PC2.

We have a range of domain specific antibodies for this target. For a full list please see all Proprotein Convertase 2 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 70 kDa. But glycosylation and other post-translational modifications make the pre-pro PC2 run at 75 kDa, the mature PC2 at 71 kDa and carboxy-processed PC2 at 66 kDa. Smaller cleavage products are also detected. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secretory vesicle. Localized in the secretion granules.

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like

Relevance 

PC2 was initially found as an insulin processing enzyme, and was the third of the human prohormone convertasae enzymes to be discovered. Like furin, PC1 and PACE4, PC2 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs ( s ubtilisin-like p roprotein c onvertases), with PC2 getting the SPC2 moniker. PC2 structure contains a propeptide domain, a catalytic domain and an RGD containing cystein-rich domain (homo-B). The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC2 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage. PC2 resides along with PC1, mainly in the dense core secretory vesicles and the TGN, and is cycled to the surface, with some of the PC2 being secreted from the cells. PC2 has been reported to be elevated in tumor cell lines and to increase tumor progression. PC2 is expressed at low levels in most tissues, but found in highest concentration in the brain, the anterior pituitary, pancreatic islet cells, thyroid, parathyroid and gut. PC2 cleaves a wide variety of proteins, including growth factors, viral coat proteins, matrix metalloproteinases, mostly after PC1 cleavage. The maturation of PC2 appears to require association with the 7B2 protein, which both helps fold PC2 properly and keeps the PC2 latent until the 7B2 protein is clipped by a PC enzyme.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

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