| | | | Immunocytochemistry/ Immunofluorescence-Proprotein convertase PC4 antibody - Aminoterminal end(ab28573)
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| Product Name | Proprotein convertase PC4 antibody - Aminoterminal end |
| | See all Proprotein convertase PC4 antibodies (2)... |
| Product type | Primary antibodies |
| Description | Rabbit polyclonal to Proprotein convertase PC4 - Aminoterminal end |
| Immunogen | Synthetic peptide corresponding to the the amino end of mature human PC4.
(Peptide available as ab41410.) |
| Reacts with
(species key) | Hu |
| Specificity | ab28573 recognises the Amino end of mature enzyme PC4. We have a range of domain specific antibodies for this target. For a full list please see all Proprotein convertase PC4 antibodies |
| Tested applications
(see key) | ICC/IF, WB |
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| Application notes
(see key) | Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 83 kDa but shed PC4 is seen as a 54 kDa band on reduced Western blots with smaller bands seen as the PC4 is further processed. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Cellular localization | Membrane; single pass membrane protein |
| Research areas | Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
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| Relevance | PC4 was the fifth of the human prohormone convertasae enzymes to be discovered. Like furin, PC1, PC2 and PACE4, PC4 is a serine proteinase that cleaves after pairs of basic amino acids, but with some differences in substrate specificity and distribution. The catalytic domain has homology to other PC enzymes, the prohormone convertase family initially discovered for their role in processing POMC, insulin and other pro-hormones, and later found to process a wider range of precursor proteins. Some have renamed the prohormone convertases as proprotein convertases, and another group has renamed the entire family with a uniform nomenclature of SPCs ( s ubtilisin-like p roprotein c onvertases). PC4 structure contains a propeptide domain, a catalytic domain and an RGD containing cystein-rich domain (homo-B). The PCs have an RxxR consensus cleavage requirement, and the propeptide is separated from the mature protein by just such a sequence. After cleavage of the propeptide domain, PC4 becomes a two-chain form, and the propeptide piece is released after a second internal cleavage. |
| | Alternative names for Proprotein convertase PC4 antibody - Aminoterminal end (ab28573) |
| Database links | The links below go to external sites and will open in a new browser window |
| Raised in | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Immunogen affinity purified |
| Storage buffer | Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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| Form | Liquid |
| Concentration | 1.000 mg/ml |
| Storage instructions | Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles. |
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Proprotein convertase PC4 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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| See below for Proprotein convertase PC4 antibody - Aminoterminal end images, references, products related to ab28573 and other tools. |
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Proprotein convertase PC4 antibody - Aminoterminal end images: |
 Immunocytochemistry/ Immunofluorescence-Proprotein convertase PC4 antibody - Aminoterminal end(ab28573)
ICC/IF image of ab28573 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28573, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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| Search PubMed (MEDLINE) for references to Proprotein convertase PC4 |
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