Overview

  • Product nameAnti-ProSAAS antibody
    See all ProSAAS primary antibodies
  • Description
    Mouse monoclonal to ProSAAS
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 173-261 of Human PCSK1N with proprietary 26 kDa tag (NP_037403).

  • Positive control
    • PCSK1N transfected 293T cell line lysate; Human cerebellum tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab118352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml.

Target

  • FunctionMay function in the control of the neuroendocrine secretory pathway. Proposed be a specific endogenous inhibitor of PCSK1. ProSAAS and Big PEN-LEN, both containing the C-terminal inhibitory domain, but not the further processed peptides reduce PCSK1 activity in the endoplasmic reticulum and Golgi. It reduces the activity of the 84 kDa form but not the autocatalytically derived 66 kDa form of PCSK1. Subsequent processing of proSAAS may eliminate the inhibition. Slows down convertase-mediated processing of proopiomelanocortin and proenkephalin. May control the intracellular timing of PCSK1 rather than its total level of activity. The function of the processed secreted peptides is not known.
  • Tissue specificityExpressed in brain and pancreas.
  • DomainProSAAS(1-180) increases secretion of enzymatically inactive PCSK1.
    The C-terminal inhibitory domain is involved in inhibtion of PCSK1. It corresponds to the probable processing intermediate Big PEN-LEN, binds to PCSK1 in vitro and contains the hexapeptide L-L-R-V-K-R, which, as a synthetic peptide, is sufficient for PCSK1 inhibition.
  • Post-translational
    modifications
    Proteolytically cleaved in the Golgi.
    O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted. Golgi apparatus > trans-Golgi network. A N-terminal processed peptide, probably Big SAAS or Little SAAS, is accumulated in cytoplasmic protein tau deposits in frontotemporal dementia and parkinsonism linked to chromosome 17 (Pick disease), Alzheimer disease and amyotrophic lateral sclerosis-parkinsonism/dementia complex 1.
  • Information by UniProt
  • Database links
  • Alternative names
    • b-LEN antibody
    • b-PEN-LEN antibody
    • b-SAAS antibody
    • Big LEN antibody
    • granin like neuroendocrine peptide antibody
    • l-LEN antibody
    • l-SAAS antibody
    • N-proSAAS antibody
    • OTTHUMP00000032426 antibody
    • PCSK1_HUMAN antibody
    • Pcsk1n antibody
    • pro-SAAS antibody
    • Proprotein convertase 1 inhibitor antibody
    • Proprotein convertase subtilisin/kexin type 1 inhibitor antibody
    • PROSAAS antibody
    • SAAS antibody
    • SAAS CT(1-49) antibody
    • SAAS CT(25-40) antibody
    see all

Anti-ProSAAS antibody images

  • Anti-ProSAAS antibody (ab118352) at 5 µg/ml + Immunizing peptide at 0.2 µg

    Predicted band size : 27 kDa
  • All lanes : Anti-ProSAAS antibody (ab118352) at 5 µg/ml

    Lane 1 : PCSK1N transfected 293T cell line lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 27 kDa
  • ab118352, at 3 µg/ml staining PCSK1N in formalin-fixed, paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
  • Detection limit for ab118352 is 0.1 ng/ml as a capture antibody.

References for Anti-ProSAAS antibody (ab118352)

ab118352 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"