Overview

  • Product name
  • Description
    Rabbit polyclonal to PROSAPIP1
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 284-380 (KSGSSSSMGR PGHLGSGEGG GGGLPFAACS PPSPSALIQE LEERLWEKEQ EVAALRRSLE QSEAAVAQVL EERQKAWERE LAELRQGCSG KLQQVAR) of Human PROSAPIP1.

  • Positive control
    • Human urinary bladder tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similarities
    Belongs to the PROSAPIP1 family.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cytoplasm > cytoskeleton. Detected at synapses, postsynaptic density, synaptic spines and dendrites.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0552 antibody
    • OTTHUMP00000030091 antibody
    • PRIP1_HUMAN antibody
    • Proline rich synapse associated protein interacting protein 1 antibody
    • ProSAP-interacting protein 1 antibody
    • ProSAPiP1 antibody
    • ProSAPiP1 protein antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab122147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122147, at 1/125, staining PROSAPIP1 in paraffin-embedded Human urinary bladder by Immunohistochemistry.

References

ab122147 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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