Anti-Prostaglandin dehydrogenase 1 antibody (ab96732)

Overview

  • Product nameAnti-Prostaglandin dehydrogenase 1 antibody
    See all Prostaglandin dehydrogenase 1 primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin dehydrogenase 1
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 255-266 of Prostaglandin dehydrogenase 1

  • Positive control
    • 293T cell lysate, A431 cell lysate, HeLaS3 cell lysate, HepG2 cell lysate, Molt-4 cell lysate, Raji cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab96732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

Target

  • FunctionProstaglandin inactivation. Contributes to the regulation of events that are under the control of prostaglandin levels. Catalyzes the NAD-dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoxin A4. Inhibits in vivo proliferation of colon cancer cells.
  • Tissue specificityDetected in colon epithelium (at protein level).
  • Involvement in diseaseDefects in HPGD are the cause of primary hypertrophic osteoathropathy autosomal recessive (PHOAR) [MIM:259100]; also known as pachydermoperiostosis autosomal recessive. Primary hypertrophic osteoarthropathy is characterized by digital clubbing, osterarthropathy, variable features of pachydermia, delayed closure of the fontanels, and congenital heart disease.
    Defects in HPGD are the cause of cranioosteoarthropathy (COA) [MIM:259100]. Clinical features include infantile onset of swelling of the joints, digital clubbing, hyperhidrosis, delayed closure of the fontanels, periostosis, and variable patent ductus arteriosus. Pachydermia is not a prominent feature.
    Defects in HPGD are a cause of isolated congenital nail clubbing (ICNC) [MIM:119900]; also called clubbing of digits or hereditary acropachy. ICNC is a rare genodermatosis characterized by enlargement of the nail plate and terminal segments of the fingers and toes, resulting from proliferation of the connective tissues between the nail matrix and the distal phalanx. It is usually symmetrical and bilateral (in some cases unilateral). In nail clubbing usually the distal end of the nail matrix is relatively high compared to the proximal end, while the nail plate is complete but its dimensions and diameter more or less vary in comparison to normal. There may be different fingers and toes involved to varying degrees. Some fingers or toes are spared, but the thumbs are almost always involved.
  • Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 hydroxyprostaglandin dehydrogenase [NAD+] antibody
    • 15 PGDH antibody
    • 15-hydroxyprostaglandin dehydrogenase [NAD+] antibody
    • 15-PGDH antibody
    • 15PGDH antibody
    • Hpgd antibody
    • Hydroxyprostaglandin dehydrogenase 15 (NAD) antibody
    • NAD+ dependent 15 hydroxyprostaglandin dehydrogenase antibody
    • OTTHUMP00000218960 antibody
    • OTTHUMP00000219016 antibody
    • OTTHUMP00000219018 antibody
    • PGDH antibody
    • PGDH_HUMAN antibody
    • PGDH1 antibody
    • PHOAR1 antibody
    • Prostaglandin dehydrogenase 1 antibody
    • SDR36C1 antibody
    • Short chain dehydrogenase/reductase family 36C member 1 antibody
    see all

Anti-Prostaglandin dehydrogenase 1 antibody images

  • Anti-Prostaglandin dehydrogenase 1 antibody (ab96732) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 29 kDa
  • ab96732, at 1/500 dilution, staining prostagladin dehydrogenase 1 in Human MDA-MB-468 xenograft by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab96732 staining Prostaglandin dehydrogenase 1 in MCF7 cells treated with diclofenac sodium salt (ab120621), by ICC/IF. Increase in Prostaglandin dehydrogenase 1 expression correlates with increased concentration of diclofenac sodium salt as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120621 (diclofenac sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96732 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
  • ab96732, at 1/200 dilution, staining prostagladin dehydrogenase 1 by immunofluorescence analysis of paraformaldehyde-fixed HeLa cells.

References for Anti-Prostaglandin dehydrogenase 1 antibody (ab96732)

ab96732 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Mouse Tissue sections (mouse skin)
Specification mouse skin
Permeabilization Yes - Tween-20
Fixative Formaldehyde
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Submitted Jan 14 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (mMSC)
Specification mMSC
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Dec 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"