Anti-Prostaglandin E Receptor EP1 antibody (ab183073)

Overview

  • Product name
    Anti-Prostaglandin E Receptor EP1 antibody
    See all Prostaglandin E Receptor EP1 primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin E Receptor EP1
  • Host species
    Rabbit
  • Specificity
    ab183073 detects endogenous level of total Prostaglandin E Receptor EP1 protein.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Prostaglandin E Receptor EP1 aa 399-402. The exact sequence is proprietary. Genbank: NP_000946
    Sequence:

    LSHF


    Database link: P34995

  • Positive control
    • 293T cell lysate, Human lymphoma tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab183073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/450 - 1/5000. Predicted molecular weight: 42 kDa.

Target

  • Relevance
    PTGER1 is a subtype 1 receptor for prostaglandin E2 (PGE2). This receptor is coupled to the phosphatidylinositol-calcium second messenger system by G(q) proteins. PTGER1 may be an important modulator of renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues.
  • Cellular localization
    Cell Membrane; multi-pass membrane protein.
  • Database links
  • Alternative names
    • EP1 antibody
    • PGE receptor EP1 subtype antibody
    • PGE2 receptor EP1 subtype antibody
    • Prostaglandin E receptor 1 subtype EP1 42kDa antibody
    • Prostaglandin E receptor 1 subtype EP1 antibody
    • Prostaglandin E2 receptor EP1 subtype antibody
    • Prostanoid EP1 receptor antibody
    • PTGER1 antibody
    see all

Images

  • All lanes : Anti-Prostaglandin E Receptor EP1 antibody (ab183073) at 1/450 dilution

    Lane 1 : Human lymphoma tissue lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 42 kDa


    Exposure time: 1 minute


    10%SDS-PAGE

References

ab183073 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (Pooled endometrial tissue)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Pooled endometrial tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Jun 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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