Recombinant
RabMAb

Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)] (ab167171)

Overview

  • Product name
    Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)]
    See all Prostaglandin E Receptor EP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR8030(B)] to Prostaglandin E Receptor EP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to a cytoplasmic region near the C terminal of Human Prostaglandin E Receptor EP2 (UniProt: P43116).

  • Positive control
    • Human placenta, Human fetal lung, JAR and WI38 cell lysates; Human colon tissue. This antibody gave a positive result when used in the following methanol fixed cell lines: SV40LT-SMC
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab167171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/250 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/80 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • ab167171 staining Prostaglandin E Receptor EP2 in the WI-38 (Human Fetal Lung Fibroblast) cell line by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/80. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • ab167171 staining Prostaglandin E Receptor EP2 in WI-38 (Human Fetal Lung Fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab167171 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • ab167171 staining Prostaglandin E Receptor EP2 in human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • All lanes : Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : MDBK (cow kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : WI-38 (human lung) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • ab167171 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab167171 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Prostaglandin E Receptor EP2 antibody [EPR8030(B)] (ab167171) at 1/1000 dilution

    Lane 1 : Human placenta lysates
    Lane 2 : Human fetal lung lysates
    Lane 3 : JAR cell lysates
    Lane 4 : WI38 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 40 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Prostaglandin E Receptor EP2 with ab167171 at 1/250 dilution.

References

This product has been referenced in:
  • Matsubara S  et al. Heterodimerization of the prostaglandin E2 receptor EP2 and the calcitonin receptor CTR. PLoS One 12:e0187711 (2017). Read more (PubMed: 29095955) »
  • Kobayashi Y  et al. Regulation of bovine oviductal NO synthesis by follicular steroids and prostaglandins. Reproduction 151:577-87 (2016). Read more (PubMed: 26940101) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Abdominal endometriotic lesion)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
15 µg
Specification
Abdominal endometriotic lesion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

Submitted Mar 21 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Bos taurus Tissue sections (oviduct, epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 citrate buffer
Permeabilization
No
Specification
oviduct, epithelium
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 01 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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