Anti-Prostaglandin E Receptor EP4 antibody (SureLight® Allophycocyanin) (ab92763)

Overview

  • Product nameAnti-Prostaglandin E Receptor EP4 antibody (SureLight® Allophycocyanin)
    See all Prostaglandin E Receptor EP4 primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin E Receptor EP4 (SureLight® Allophycocyanin)
  • ConjugationSureLight® Allophycocyanin. Ex: 652nm, Em: 657nm
  • Specificityab92763 is reactive with EP4 receptor and non-reactive with EP1, EP2, and EP3 receptors.
  • Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
    Predicted to work with: Rabbit, Cow, Chimpanzee, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    GSGRAGPAPKGSSLQVTFPSETLNLSEKCI

    , corresponding to C terminal amino acids 459-488 of Human Prostaglandin E Receptor EP4

Properties

Applications

Our Abpromise guarantee covers the use of ab92763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 3 µg/ml.

Target

Anti-Prostaglandin E Receptor EP4 antibody (SureLight® Allophycocyanin) images

  • ICC/IF image of ab92763 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92763, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Prostaglandin E Receptor EP4 antibody (SureLight® Allophycocyanin) (ab92763)

ab92763 has not yet been referenced specifically in any publications.

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The peptide sequence used to raise the anti-Prostaglandin E Receptor EP4 antibody (ab92763) is SLRTQDATQTSCSTQSDASKQADL and the sequence for the ...

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