Anti-Prostaglandin E Synthase antibody (ab23727)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab23727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). Extensive washing of the transfer membrane at each step to reduce non-specific background binding effects is required. Starting exposure time of 45 seconds is suggested. Lower dilutions of antibody should be used for alkaline phosphatase detection.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).
    • Sequence similaritiesBelongs to the MAPEG family.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Glutathione S transferase 1 like 1 antibody
      • MGC10317 antibody
      • MGST IV antibody
      • MGST1 L1 antibody
      • MGST1 like 1 antibody
      • MGST1-L1 antibody
      • MGST1L1 antibody
      • MGSTIV antibody
      • Microsomal glutathione S transferase 1 like 1 antibody
      • Microsomal glutathione S-transferase 1-like 1 antibody
      • Microsomal prostaglandin E synthase 1 antibody
      • Microsomal prostaglandin E synthase 1 antibody
      • mPGES 1 antibody
      • MPGES antibody
      • mPGES-1 antibody
      • p53 induced apoptosis protein 12 antibody
      • p53 induced gene 12 antibody
      • p53 induced gene 12 protein antibody
      • p53-induced gene 12 protein antibody
      • PGES antibody
      • PIG 12 antibody
      • PIG12 antibody
      • PP 102 antibody
      • PP 1294 antibody
      • PP102 antibody
      • PP1294 antibody
      • Prostaglandin E synthase antibody
      • PTGES antibody
      • PTGES_HUMAN antibody
      • TP53I12 antibody
      • Tumor protein p53 inducible protein 12 antibody
      see all

    Anti-Prostaglandin E Synthase antibody images

    • All lanes : Anti-Prostaglandin E Synthase antibody (ab23727) at 1/1000 dilution

      Lane 1 : Vector transfected HCT116 cell lysate
      Lane 2 : Prostaglandin E Synthase transfected HCT116 cell lysate

      Developed using the ECL technique

      Predicted band size : 17 kDa
      Observed band size : 17 kDa


      Exposure time : 2 hours

    References for Anti-Prostaglandin E Synthase antibody (ab23727)

    ab23727 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount 20 µg
    Specification NIH 3T3 cells
    Treatment 24 hour IL-1b stimulation (1ng/ml)
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 10 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A549 cells)
    Loading amount 20 µg
    Specification A549 cells
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 10 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"