Anti-Prostaglandin E Synthase antibody (ab62050)

Overview

  • Product nameAnti-Prostaglandin E Synthase antibody
    See all Prostaglandin E Synthase primary antibodies
  • Description
    Rabbit polyclonal to Prostaglandin E Synthase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to 14 amino acids near the N-terminus of Human Prostaglandin E Synthase.

  • Positive control
    • Human testis tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionCatalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).
  • Sequence similaritiesBelongs to the MAPEG family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glutathione S transferase 1 like 1 antibody
    • MGC10317 antibody
    • MGST IV antibody
    • MGST1 L1 antibody
    • MGST1 like 1 antibody
    • MGST1-L1 antibody
    • MGST1L1 antibody
    • MGSTIV antibody
    • Microsomal glutathione S transferase 1 like 1 antibody
    • Microsomal glutathione S-transferase 1-like 1 antibody
    • Microsomal prostaglandin E synthase 1 antibody
    • Microsomal prostaglandin E synthase 1 antibody
    • mPGES 1 antibody
    • MPGES antibody
    • mPGES-1 antibody
    • p53 induced apoptosis protein 12 antibody
    • p53 induced gene 12 antibody
    • p53 induced gene 12 protein antibody
    • p53-induced gene 12 protein antibody
    • PGES antibody
    • PIG 12 antibody
    • PIG12 antibody
    • PP 102 antibody
    • PP 1294 antibody
    • PP102 antibody
    • PP1294 antibody
    • Prostaglandin E synthase antibody
    • PTGES antibody
    • PTGES_HUMAN antibody
    • TP53I12 antibody
    • Tumor protein p53 inducible protein 12 antibody
    see all

Anti-Prostaglandin E Synthase antibody images

  • ab62050 staining Prostaglandin E Synthase in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton-X and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 20 hours at 4°C. ab150075, a donkey anti-rabbit Alexa Fluor® 647- (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, staining Prostaglandin E Synthase with ab62050 at 10 µg/ml.

References for Anti-Prostaglandin E Synthase antibody (ab62050)

ab62050 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample Mouse Tissue sections (Testis)
Specification Testis
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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Abcam user community

Verified customer

Submitted Dec 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH3T3 cells)
Loading amount 20 µg
Specification NIH3T3 cells
Treatment 24 hours IL1b stimulation (1ng/ml)
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Jun 10 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549 cells)
Loading amount 20 µg
Specification A549 cells
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"