|Sample type||Average %||Range|
|Saliva||123.3||% - %|
|Urine||108.9||% - %|
|Whole Blood||101.2||% - %|
|Tissue Culture Media||104.4||% - %|
Abcam’s Prostaglandin E2 (PGE2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine and tissue culture media and other biological fluids.
A mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Prostaglandin E2 antibody. After incubation the excess reagents are washed away and pNpp substrate is added and is catalyzed by AP to produce a yellow color. The intensity of the yellow coloration is inversely proportional to the amount of Prostaglandin E2 captured in the plate.
This kit is unsuitable for mouse serum as samples containing mouse IgG may interfere with the assay.
Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
|Compound||% Cross Reactivity|
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 30ml|
|Assay Buffer||1 x 30ml|
|Goat anti-mouse IgG Microplate (12 x 8 wells)||1 unit|
|PGE2 Alkaline Phosphatase Conjugate||1 x 6ml|
|Plate Sealer||1 unit|
|pNpp Substrate||1 x 20ml|
|Prostaglandin E2 Antibody||1 x 6ml|
|Prostaglandin E2 Standard||1 x 500µl|
|Stop Solution||1 x 5ml|
Our Abpromise guarantee covers the use of ab133021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|