For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
|Sample type||Average %||Range|
|Saliva||123.3||% - %|
|Urine||108.9||% - %|
|Whole Blood||101.2||% - %|
|Tissue Culture Media||104.4||% - %|
Abcam’s Prostaglandin E2 (PGE2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine and tissue culture media and other biological fluids.
A mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Prostaglandin E2 antibody. After incubation the excess reagents are washed away and pNpp substrate is added and is catalyzed by AP to produce a yellow color. The intensity of the yellow coloration is inversely proportional to the amount of Prostaglandin E2 captured in the plate.
Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.
|Compound||% Cross Reactivity|
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 30ml|
|Assay Buffer||1 x 30ml|
|Goat anti-mouse IgG Microplate (12 x 8 wells)||1 unit|
|PGE2 Alkaline Phosphatase Conjugate||1 x 6ml|
|Plate Sealer||1 unit|
|pNpp Substrate||1 x 20ml|
|Prostaglandin E2 Antibody||1 x 6ml|
|Prostaglandin E2 Standard||1 x 500µl|
|Stop Solution||1 x 5ml|
Our Abpromise guarantee covers the use of ab133021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"