Overview

  • Product name
    Prostaglandin E2 ELISA Kit
    See all PGE2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low PGE2 24 116pg/ml 8.9%
    Medium PGE2 24 492pg/ml 5.8%
    High PGE2 24 2416pg/ml 17.5%
    Inter-assay
    Sample n Mean SD CV%
    Low PGE2 8 111pg/ml 3%
    Medium PGE2 8 419pg/ml 5.1%
    High PGE2 8 1902pg/ml 3.9%
  • Sample type
    Saliva, Urine, Serum, Plasma, Tissue, Whole Blood, Cell Lysate
  • Assay type
    Competitive
  • Sensitivity
    13.4 pg/ml
  • Range
    39.1 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 123.3 % - %
    Urine 108.9 % - %
    Whole Blood 101.2 % - %
    Tissue Culture Media 104.4 % - %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Species independent
  • Product overview

     Abcam’s Prostaglandin E2 (PGE2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prostaglandin E2 in serum, saliva, urine and tissue culture media and other biological fluids.


    A mouse IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Prostaglandin E2 antibody. After incubation the excess reagents are washed away and pNpp substrate is added and is catalyzed by AP to produce a yellow color. The intensity of the yellow coloration is inversely proportional to the amount of Prostaglandin E2 captured in the plate.


    This kit is unsuitable for mouse serum as samples containing mouse IgG may interfere with the assay.

  • Notes

    Prostaglandin E2 (PGE2) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has been shown to have a number of biological actions, including vasodilation, both anti- and proinflammatory action, modulation of sleep/wake cycles, and facilitation of the replication of human immunodeficiency virus. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

    Cross Reactivity

    Compound % Cross Reactivity
    PGE2 100
    PGE1 70
    PGE3 16.3
    PGF 1.4
    PGF 0.7
    6-keto-PGF 0.6
    PGA2 0.1
    PGB1 0.1
    13,14-dihydro-15-keto-PGF <0.1
    6,15-keto-13,14-dihydro-PGF <0.1
    Thromboxane B2 <0.1
    2-Arachidonoylglycerol <0.1
    Anandamide <0.1
    PGD2 <0.1
    Arachadonic Acid <0.1
  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab133021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted
  • Prostaglandin E2 measured in biological fluids and cell culture supernatants showing quantity (pg) per mL of tested sample. Samples were diluted 1-9 fold.

Protocols

References

This product has been referenced in:
  • Russo L  et al. Liver-specific rescuing of CEACAM1 reverses endothelial and cardiovascular abnormalities in male mice with null deletion of Ceacam1 gene. Mol Metab 9:98-113 (2018). Read more (PubMed: 29396368) »
  • Wang B  et al. Chronic administration of parecoxib exerts anxiolytic-like and memory enhancing effects and modulates synaptophysin expression in mice. BMC Anesthesiol 17:152 (2017). Read more (PubMed: 29132299) »

See all 9 Publications for this product

Customer reviews and Q&As

Filter by Ratings

GOOD

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Sample: Mouse Cell (Homogenised lung tissue)
Specification: Homogenised lung tissue
Type of Kit Used: ELISA
Positive control: Virally infected lung tissue.
Did you use the Abcam Kit protocol?: Yes
Additional data
Additional Notes: Overall, a good kit.
Username

Abcam user community

Verified customer

Submitted Jan 27 2016

Abreviews
PGE2 human Kit works fine and recognices human PGE2 with no doubts.
It's very easy to use.

As manual says that could recognice mouse samples we've test this possibility with mouse samples and, in our hands, the replica signal is strong enough to be agree with this statment.

See attached picture in which we show standard curve and a simple bar with levels of PGE2 in mouse MSC supenantants.
Username

Abcam user community

Verified customer

Submitted Jul 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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