Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Prostaglandin J2 images
Functional Studies-Prostaglandin J2(ab120913)
ab80579 staining tau in SKNSH cells treated with prostaglandin J2 (ab120913), by ICC/IF. Expression of tau expression is restringed to the perinuclear zone with increased concentration of prostaglandin J2, as described in literature. The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120913 (prostaglandin J2) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab80579 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei (blue) were counterstained with DAPI and membrane is was stained using WGA (red).
References for Prostaglandin J2 (ab120913)
This product has been referenced in:
Arnaud LT et al. Proteasome-caspase-cathepsin sequence leading to tau pathology induced by prostaglandin J2 in neuronal cells. J Neurochem110:328-42 (2009).
Read more (PubMed: 19457109) »
Kliewer SA et al. A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor gamma and promotes adipocyte differentiation. Cell83:813-9 (1995).
Read more (PubMed: 8521498) »