Anti-Prostate Specific Antigen antibody [5A6] (ab10185)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody [5A6]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [5A6] to Prostate Specific Antigen
  • SpecificityEquimolar total PSA, epitope 5. No cross reactivity for human kallikrein 2.
  • Tested applicationsSuitable for: ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human PSA antigen purified from seminal plasma.

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [2H9] to PSA (ab10184), and Mouse monoclonal [8A6] to PSA (ab10187).
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187). best pair for the detection of free PSA: (detection ab - capture ab) ab10187 - ab10185 For the detection of total PSA ab10185 can be used as capture antibody in conjunction with ab10184. For the detection of total PSA ab10185 can be used as a detection antibody in conjugation with ab10186.

Target

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

References for Anti-Prostate Specific Antigen antibody [5A6] (ab10185)

ab10185 has not yet been referenced specifically in any publications.

Product Wall

Yes, it is possible to use this pair in either combination.


I have been in contact with the lab and unfortunately we cannot split our products into smaller sizes for logistical reasons concerning storage therefore, we could only supply you with the sizes indicated on the datasheet.

Thank you for contacting us. None of these products contain BSA. We do have the storage buffer listed on the datasheets for your convenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

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Thank you for contacting us.

I have been in contact with the lab regarding light chain specificity as you have requested. Unfortunately the light chain specificity for ab10185 has not been determined. We apologize for any inconvenience tha...

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Thank you for your enquiry.

We have used our internal sandwich ELISA protocol only to determine which monoclonal antibodies are working together as pairs. Usually any general protocol, like for example the one youfind below, with proper opti...

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Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

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The cost of the HRP conjugate, if we are able to add one that is compatible with the ab10185 clone (5A6), would be about USD$335. We do have the ab10185 clone (5A6) conjugated to HRP, if you are interested in trying this as the detection antibody ra...

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Thank you for your interest in our products. We unfortunately do not have a PSA protein for a positive control but you may be able to find a source by consulting Biocompare.com. Regarding a detection antibody conjugated to HRP, we do not at the moment ...

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Thank you for your email. I have instructed our accounting department to issue you a credit for your order for ab10185 and ab10186. If you have any questions regarding the credit, please contact accounts@abcam.com If I can be of additional assistanc...

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Thank you very much for your patience. I contacted our source for ab10185 and ab10186 and they provided me with the following information which should help you. PSA can be used as an antigen adsorbed on plates in ELISA only in following conditions:...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"