Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466)


  • Product name
    Anti-Prostate Specific Antigen antibody [5A6] (HRP)
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP)
  • Conjugation
  • Specificity
    Equimolar total PSA, epitope 5. No cross reactivity for human kallikrein 2.
  • Tested applications
    Suitable for: Sandwich ELISA, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human PSA antigen purified from semenal plasma).

  • Positive control
    • This antibody gave a postive signal in Human Prostate tissue lysate and LNCaP whole cell lysate.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab24466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [8A6] to Prostate Specific Antigen (ab10187).

For the detection of free PSA, ab24466 can be used as a detection antibody in conjugation with ab10187. For total PSA detection ab24466 can be used as a capture antibody and ab10184 as a detection antibody.

WB 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).
ELISA Use at an assay dependent concentration.


  • Function
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all


  • All lanes : Anti-Prostate Specific Antigen antibody [5A6] (HRP) (ab24466) at 1/5000 dilution

    Lane 1 : Human prostate tumor tissue lysate (adenocarcinoma) - total protein (ab30305)
    Lane 2 : LNCaP nuclear extract lysate (ab14857)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes


This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

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Thank you for contacting Abcam.

It appears our datasheet for ab24466 incorrectly states that 0.1% sodium azide is included in the storage buffer. Sodium azide is not present in the storage buffer since, as you mentioned, sodium azide affects H...

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That is correct: ab10184 for capture and ab24466 for detection. These are two different clones.  The detection antibody, ab24466, is conjugated to HRP. Some of the catalogue numbers of our other PSA antibodies are very similar so I will not even mentio...

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Thank you for your inquiry. Unfortunately, the isoelectric points of these antibodies have not been determined. According to my research, the pI of most monoclonal antibodies is around 6.1. The isoelectric point for horseradish peroxidase isozyme...

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