Anti-Prostate Specific Antigen antibody [5G6] (ab10186)

Overview

  • Product name
    Anti-Prostate Specific Antigen antibody [5G6]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [5G6] to Prostate Specific Antigen
  • Specificity
    Specific for total PSA and epitope 4. No cross reactivity with human kallikrein 2.
  • Tested applications
    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human PSA antigen purified from semenal plasma

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab76113 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab10186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466). For the detection of total PSA ab10186 can be used as a capture antibody in conjugation with ab10185. For the detection of total PSA ab10186 can be used as a capture antibody in conjugation with ab24466.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185).

Target

  • Function
    Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similarities
    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

References

This product has been referenced in:
  • Xie C & Wang G Development of simultaneous detection of total prostate-specific antigen (tPSA) and free PSA with rapid bead-based immunoassay. J Clin Lab Anal 25:37-42 (2011). Read more (PubMed: 21254241) »
  • Escamilla-Gómez V  et al. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron 24:2678-83 (2009). Human . Read more (PubMed: 19261459) »

See all 2 Publications for this product

Customer reviews and Q&As


I have been in contact with the lab and unfortunately we cannot split our products into smaller sizes for logistical reasons concerning storage therefore, we could only supply you with the sizes indicated on the datasheet.

Thank you for contacting us. None of these products contain BSA. We do have the storage buffer listed on the datasheets for your convenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

Read More

Thank you for your enquiry. The concentration of this antibody is 9.40 mg/ml. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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