Mouse monoclonal  to Prostate Specific Antigen
Human prostate-specific antigen - 100%
PSA/alpha-1-ACT-complex - 59%
Kallikrein - <0.04%
PAP - <0.04%
Cross-reactivity values have been calculated on weight/weight basis using 125-I-labelled human human prostate-specific antigen in assay. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays.
Affinity constant: 1x1011 l/mol human prostate-specific antigen.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependant concentration.
IHC-P: Use at an assay dependent dilution.
RIA: Use at an assay dependant concentration.
Nanowires array: 1/50 - 1/500.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.
Human normal prostate. Staining is localised as cytoplasmic and secreted. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system, at room temperature: sections were rehydrated and antigen retrieved with buffers (citrate pH 6.1) in a PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Thaxton CS et al. Nanoparticle-based bio-barcode assay redefines "undetectable" PSA and biochemical recurrence after radical prostatectomy. Proc Natl Acad Sci U S A106:18437-42 (2009).
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