Anti-Prostate Specific Antigen antibody [8A6] (ab10187)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody [8A6]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [8A6] to Prostate Specific Antigen
  • SpecificitySpecific for free PSA, epitope 1. No cross reactivity with human kallikrein 2.
  • Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human PSA antigen purified from semenal plasma.

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185) and Mouse monoclonal [5A6] to Prostate Specific Antigen (HRP) (ab24466). Best pair for detection of free PSA - (detection ab - capture ab) ab10187 - ab10185 For the detection of free PSA ab10187 can be used as a detection antibody in conjugation with ab10184. For the detection of free PSA ab10187 can be used as a detection antibody in conjugation with ab24466.
ELISA Use at an assay dependent dilution. Can be paired for ELISA with Mouse monoclonal [2H9] to Prostate Specific Antigen (ab10184) and Mouse monoclonal [5A6] to Prostate Specific Antigen (ab10185).

Target

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

References for Anti-Prostate Specific Antigen antibody [8A6] (ab10187)

This product has been referenced in:
  • Xie C & Wang G Development of simultaneous detection of total prostate-specific antigen (tPSA) and free PSA with rapid bead-based immunoassay. J Clin Lab Anal 25:37-42 (2011). Read more (PubMed: 21254241) »
  • Escamilla-Gómez V  et al. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron 24:2678-83 (2009). Human . Read more (PubMed: 19261459) »

See all 2 Publications for this product

Product Wall

Yes, it is possible to use this pair in either combination.

Thank you for contacting us. None of these products contain BSA. We do have the storage buffer listed on the datasheets for your convenience.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more ...

Read More

Thank you for your enquiry.

We have used our internal sandwich ELISA protocol only to determine which monoclonal antibodies are working together as pairs. Usually any general protocol, like for example the one youfind below, with proper opti...

Read More

Thank you for your reply.

I have reviewed the details of this experiment and have the following feedback:

1. ab119154 Prostate Specific Antigen protein is full length human protein, so should be detected by both ab77310 and ab1028...

Read More

Thank you for your enquiry. Specificity was tested in ELISA with free PSA or PSA-ACT complex as antigen. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"