Anti-Prostate Specific Antigen antibody (ab111858)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody
    See all Prostate Specific Antigen primary antibodies
  • Description
    Rabbit polyclonal to Prostate Specific Antigen
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Prostate Specific Antigen.

  • Positive control
    • This antibody gave a positive signal in both Human Prostate and Testis tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab111858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).

Target

  • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
  • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen, prostate-specific antibody
    • APS antibody
    • Gamma seminoprotein antibody
    • Gamma-seminoprotein antibody
    • hK3 antibody
    • Kallikrein 3 antibody
    • Kallikrein related peptidase 3 antibody
    • Kallikrein-3 antibody
    • KLK 3 antibody
    • KLK2A1 antibody
    • Klk3 antibody
    • KLK3_HUMAN antibody
    • P-30 antigen antibody
    • P30 antigen antibody
    • Prostate-specific antigen antibody
    • Psa antibody
    • Semenogelase antibody
    • Seminin antibody
    see all

Anti-Prostate Specific Antigen antibody images

  • All lanes : Anti-Prostate Specific Antigen antibody (ab111858) at 1 µg/ml

    Lane 1 : Human prostate tissue lysate (cirrhosis) - total protein (ab30307)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 32 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-Prostate Specific Antigen antibody (ab111858)

ab111858 has not yet been referenced specifically in any publications.

Product Wall

Regarding polyclonal products there is a possibility that some IgM could be present in our final eluate. As I understand it, when a rabbit is immunized it will first generate IgMs before generating IgGs. Our immunization protocols lend themselves to Ig...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"