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Products:Neuroscience >> Cell Adhesion Proteins >> Proteases
Overview
Product name
Anti-Prostate Specific Antigen antibody - Catalytic domain
See all Prostate Specific Antigen products (32) ...
Description
Rabbit polyclonal to Prostate Specific Antigen - Catalytic domain
Specificity
This antibody is specific for Kallikrein 3, it does not react with the other Kallikreins (1,2,4-15).
Tested applications
WB, SDS-PAGEmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the catalytic domain of human Kallikrein 3. (Peptide available as ab43085.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Tumor antigens
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Adhesion Proteins >> Proteases
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Western blot - Kallikrein 3 antibody - Catalytic domain (ab40949)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 29 kDa.(1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 29 kDa. Dilution optimised using Chromogenic detection.)
SDS-PAGE: Use at an assay dependent dilution. (Glycosylation and other post translational modifications make the apparent weight on SDS PAGE gels 32-33 kDa.)
Target
Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted.
Target information above from: UniProt accessionP07288
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APS antibody
- Gamma seminoprotein antibody
- Gamma-seminoprotein antibody
see all
Database links
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
Anti-Prostate Specific Antigen antibody - Catalytic domain images:
Western blot - Kallikrein 3 antibody - Catalytic domain (ab40949)
All lanes : Anti-Prostate Specific Antigen antibody - Catalytic domain (ab40949) at 1/1000 dilution
Lane 1 : Human Kallikrein 3 at 0.05 µg
Lane 2 : Human Kallikrein 3 at 0.01 µg
Lane 3 : Human Kallikrein 3 at 0.001 µg
Predicted band size : 29 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Prostate Specific Antigen antibody - Catalytic domain (ab40949)
ab40949 has not yet been referenced specifically in any publications.
Publishing research using ab40949? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"