Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody [EP1588Y]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen
  • SpecificityThe antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of human Prostate Specific Antigen

  • Positive control
    • Human prostate carcinoma tissue lysate. Human prostate carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
    • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    Anti-Prostate Specific Antigen antibody [EP1588Y] images

    • ab76113, at 1/100 dilution, staining Prostate Specific Antigen in paraffin-embedded human prostate carcinoma tissue by immunohistochemistry.
    • Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/10000 dilution + human prostate carcinoma tissue lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

    This product has been referenced in:
    • Deng X  et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene N/A:N/A (2016). WB ; Human . Read more (PubMed: 27546619) »
    • Volpetti F  et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). Read more (PubMed: 25680117) »

    See all 6 Publications for this product

    Product Wall

    Je suis désolée d'apprendre que ab76113 ne vous donne pas de bons résultats en Western blot.

    En accord avec notre garantie et comme discuté au téléphone, nous allons vous remplacer ou rembourser cet anticorps. Veuillez pour ceci nous faire p...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"