Recombinant
RabMAb

Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

Overview

  • Product name
    Anti-Prostate Specific Antigen antibody [EP1588Y]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Rabbit monoclonal [EP1588Y] to Prostate Specific Antigen
  • Specificity
    The antibody provided good results in western blot when tested in human prostate lysate. Unfortunately, when 22RV1 cell lysate was used no band was detected and inconsistent results were obtained with LNCaP cell lysate (where different lots of cell lysate were used for the test). Please contact our Scientific Support if you have any feedback and/or questions.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminal of human Prostate Specific Antigen

  • Positive control
    • WB: Human prostate carcinoma tissue lysate. IHC-P: Human prostate carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
    • Sequence similarities
      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    Anti-Prostate Specific Antigen antibody [EP1588Y] images

    • ab76113, at 1/100 dilution, staining Prostate Specific Antigen in paraffin-embedded human prostate carcinoma tissue by immunohistochemistry.
    • All lanes : Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113) at 1/1000 dilution

      Lane 1 : Human prostate tissue lysate
      Lane 2 : LNCaP whole cell lysate
      Lane 3 : 22Rv1 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 29 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)

      Exposure time -
      Lane 1: 15 seconds.
      Lanes 2-3: 3 minutes.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Prostate Specific Antigen antibody [EP1588Y] (ab76113)

    This product has been referenced in:
    • Deng X  et al. Protein arginine methyltransferase 5 functions as an epigenetic activator of the androgen receptor to promote prostate cancer cell growth. Oncogene N/A:N/A (2016). WB ; Human . Read more (PubMed: 27546619) »
    • Volpetti F  et al. A microfluidic platform for high-throughput multiplexed protein quantitation. PLoS One 10:e0117744 (2015). Read more (PubMed: 25680117) »

    See all 6 Publications for this product

    Product Wall

    Je suis désolée d'apprendre que ab76113 ne vous donne pas de bons résultats en Western blot.

    En accord avec notre garantie et comme discuté au téléphone, nous allons vous remplacer ou rembourser cet anticorps. Veuillez pour ceci nous faire p...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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