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Anti-Prostate Specific Antigen antibody - Kallikrein loop
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Rabbit polyclonal to Prostate Specific Antigen - Kallikrein loop
This antibody reacts with human Kallikrein 3, but does not react with other human Kallikreins (K1, 2, 4-15).
Reacts with
Human
Synthetic peptide based on the Kallikrein loop area of human Kallikrein 3. (Peptide available as ab41421.)
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cancer >> Tumor biomarkers >> Tumor antigens
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Kallikreins
Cancer >> Tumor immunology >> Tumor-associated antigens
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Kallikreins
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Kallikreins
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
Neuroscience >> Cell Adhesion Proteins >> Proteases
Western blot - Kallikrein 3 antibody - Kallikrein loop (ab28563)
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Our Abpromise guarantee covers the use of ab28563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 (when using colorimetric substrates such as BCIP/NBT) - 1/5000 (for chemiluminescent substrates). Detects a band of approximately 33 kDa, due to glycosylation and other post translational modifications. (Predicted molecular weight: 29 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Secreted.
Target information above from: UniProt accessionP07288
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Kallikrein 3 antibody - Kallikrein loop (ab28563)

All lanes : Anti-Prostate Specific Antigen antibody - Kallikrein loop (ab28563) at 1/1000 dilution
Lane 1 : Kallikrein 3 at 0.05 µg
Lane 2 : Kallikrein 3 at 0.01 µg
Lane 3 : Kallikrein 3 at 0.001 µg
Predicted band size : 29 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Additional bands at : 85 kDa (possible cleavage fragment).
ab28563 has not yet been referenced specifically in any publications.
Publishing research using ab28563? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-Prostate Specific Antigen antibody - Kallikrein loop (ab28563) at 1/1000 dilution
Lane 1 : Kallikrein 3 at 0.05 µg
Lane 2 : Kallikrein 3 at 0.01 µg
Lane 3 : Kallikrein 3 at 0.001 µg
Predicted band size : 29 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Additional bands at : 85 kDa (possible cleavage fragment).
0
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