Anti-Prostate Specific Antigen antibody [PSA 28/A4] (ab49395)

Overview

  • Product nameAnti-Prostate Specific Antigen antibody [PSA 28/A4]
    See all Prostate Specific Antigen primary antibodies
  • Description
    Mouse monoclonal [PSA 28/A4] to Prostate Specific Antigen
  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Seminal fluid preparation.

  • Positive control
    • Human prostate.
  • General notes


    Hybridoma partner: Mouse myeloma (PYS).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionHydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
    • Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • antigen, prostate-specific antibody
      • APS antibody
      • Gamma seminoprotein antibody
      • Gamma-seminoprotein antibody
      • hK3 antibody
      • Kallikrein 3 antibody
      • Kallikrein related peptidase 3 antibody
      • Kallikrein-3 antibody
      • KLK 3 antibody
      • KLK2A1 antibody
      • Klk3 antibody
      • KLK3_HUMAN antibody
      • P-30 antigen antibody
      • P30 antigen antibody
      • Prostate-specific antigen antibody
      • Psa antibody
      • Semenogelase antibody
      • Seminin antibody
      see all

    Anti-Prostate Specific Antigen antibody [PSA 28/A4] images

    • Ab49395 staining human normal prostate tissue. Staining is localised to cytoplasm and extracellular space.
      Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be

    References for Anti-Prostate Specific Antigen antibody [PSA 28/A4] (ab49395)

    This product has been referenced in:
    • Zhu L  et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »
    • Usuda H & Emura I Composite paraganglioma-ganglioneuroma of the urinary bladder. Pathol Int 55:596-601 (2005). Read more (PubMed: 16143037) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (DU145 Prostate cancer cell line)
    Permeabilization Yes - 0.1% v/v TritonX-100
    Specification DU145 Prostate cancer cell line
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Dr. Dimitra Kalamida

    Verified customer

    Submitted Oct 05 2016

    Thank you for contacting us.

    The IgG concentration is 0.1mg/ml.

    Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"