Anti-Prostatic Acid Phosphatase antibody (ab116614)

Overview

  • Product nameAnti-Prostatic Acid Phosphatase antibody
    See all Prostatic Acid Phosphatase primary antibodies
  • Description
    Goat polyclonal to Prostatic Acid Phosphatase
  • Specificityab116614 is expected to recognize both reported isoforms (NP_001127666.1; NP_001090.2).
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    NHMKRATQIPSYKK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 269-282 of Human Prostatic Acid Phosphatase (NP_001127666.1; NP_001090.2).

  • Positive control
    • Human Prostate lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 48 kDa.
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Sequence similaritiesBelongs to the histidine acid phosphatase family.
  • Cellular localizationSecreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • Acid phosphatase prostate antibody
    • ACP 3 antibody
    • ACP3 antibody
    • acpP antibody
    • Ecto-5'-nucleotidase antibody
    • PAP antibody
    • PPAP_HUMAN antibody
    • Prostatic acid phosphatase antibody
    • Prostatic acid phosphotase antibody
    • Thiamine monophosphatase antibody
    • TMPase antibody
    see all

Anti-Prostatic Acid Phosphatase antibody images

  • Anti-Prostatic Acid Phosphatase antibody (ab116614) at 0.01 µg/ml + Human Prostate lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 48 kDa
  • ab116614 (3.8µg/ml) staining of paraffin embedded Human Prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland.
    Tissue Treatment: Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-Prostatic Acid Phosphatase antibody (ab116614)

ab116614 has not yet been referenced specifically in any publications.

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