Anti-Prostatic Acid Phosphatase antibody (ab166825)

Overview

  • Product name
    Anti-Prostatic Acid Phosphatase antibody
    See all Prostatic Acid Phosphatase primary antibodies
  • Description
    Goat polyclonal to Prostatic Acid Phosphatase
  • Host species
    Goat
  • Specificity
    ab166825 is expected to recognize both reported isoforms
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Monkey, Baboon, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    NHMKRATQIPSYKK

    with a Cysteine residue linker, corresponding to internal sequence amino acids 268-282 of Human Prostatic Acid Phosphatase (Uniprot: P15309).

  • Positive control
    • Human prostate tissue and lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab166825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-Prostatic Acid Phosphatase antibody (ab166825) at 0.01 µg/ml + Human prostate lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 45 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.
  • Immunohistochemical analysis of formalin fixed, paraffin embedded labeling Prostatic Acid Phosphatase with ab166825 at 3.75 µg/ml.

References

ab166825 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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