Recombinant
RabMAb

Anti-Prostatic Acid Phosphatase antibody [EPR4066] (ab108984)

Overview

  • Product name
    Anti-Prostatic Acid Phosphatase antibody [EPR4066]
    See all Prostatic Acid Phosphatase primary antibodies
  • Description
    Rabbit monoclonal [EPR4066] to Prostatic Acid Phosphatase
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide against human Prostatic Acid Phosphatase(P15309).

  • Positive control
    • Prostate cancer cell lysate, human prostatic and human prostatic adenocarcinoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Sequence similarities
      Belongs to the histidine acid phosphatase family.
    • Cellular localization
      Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • Acid phosphatase prostate antibody
      • ACP 3 antibody
      • ACP3 antibody
      • acpP antibody
      • Ecto-5'-nucleotidase antibody
      • PAP antibody
      • PPAP_HUMAN antibody
      • Prostatic acid phosphatase antibody
      • Prostatic acid phosphotase antibody
      • Thiamine monophosphatase antibody
      • TMPase antibody
      see all

    Anti-Prostatic Acid Phosphatase antibody [EPR4066] images

    • Anti-Prostatic Acid Phosphatase antibody [EPR4066] (ab108984) at 1/1000 dilution + Prostate cancer cell lysate at 10 µg

      Predicted band size : 45 kDa
    • Immunohistochemical staining of paraffin-embedded human prostatic tissue using anti-Prostatic Acid Phosphatase (ab108984).
    • Immunohistochemical staining of paraffin-embedded human prostatic adenocarcinoma tissue using anti-Prostatic Acid Phosphatase (ab108984).
    • ab108984 showing negative staining in Skeletal muscle tissue.

    • ab108984 showing negative staining in Colonic adenocarcinoma tissue.

    • ab108984 showing negative staining in Normal tonsil tissue.

    • ab108984 showing negative staining in Normal placenta tissue.

    • ab108984 showing positive staining in Prostatic carcinoma tissue.

    • ab108984 showing negative staining in Breast carcinoma tissue.

    • ab108984 showing negative staining in Cervical carcinoma tissue.

    • ab108984 showing negative staining in Normal breast tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Prostatic Acid Phosphatase antibody [EPR4066] (ab108984)

    This product has been referenced in:
    • Wei J  et al. Rapid progression of mixed neuroendocrine carcinoma-acinar adenocarcinoma of the prostate: A case report. Oncol Lett 12:1019-1022 (2016). IHC-P ; Human . Read more (PubMed: 27446387) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate Cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA based buffer pH 9.0
    Permeabilization
    No
    Specification
    Prostate Cancer
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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