Anti-Prostatic Acid Phosphatase antibody [EPR4066] (ab108984)

Overview

  • Product nameAnti-Prostatic Acid Phosphatase antibody [EPR4066]
    See all Prostatic Acid Phosphatase primary antibodies
  • Description
    Rabbit monoclonal [EPR4066] to Prostatic Acid Phosphatase
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide against human Prostatic Acid Phosphatase(P15309).

  • Positive control
    • Prostate cancer cell lysate, human prostatic and human prostatic adenocarcinoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • Sequence similaritiesBelongs to the histidine acid phosphatase family.
    • Cellular localizationSecreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • Acid phosphatase prostate antibody
      • ACP 3 antibody
      • ACP3 antibody
      • acpP antibody
      • Ecto-5'-nucleotidase antibody
      • PAP antibody
      • PPAP_HUMAN antibody
      • Prostatic acid phosphatase antibody
      • Prostatic acid phosphotase antibody
      • Thiamine monophosphatase antibody
      • TMPase antibody
      see all

    Anti-Prostatic Acid Phosphatase antibody [EPR4066] images

    • Anti-Prostatic Acid Phosphatase antibody [EPR4066] (ab108984) at 1/1000 dilution + Prostate cancer cell lysate at 10 µg

      Predicted band size : 45 kDa
    • Immunohistochemical staining of paraffin-embedded human prostatic tissue using anti-Prostatic Acid Phosphatase (ab108984).
    • Immunohistochemical staining of paraffin-embedded human prostatic adenocarcinoma tissue using anti-Prostatic Acid Phosphatase (ab108984).
    • ab108984 showing negative staining in Skeletal muscle tissue.

    • ab108984 showing negative staining in Colonic adenocarcinoma tissue.

    • ab108984 showing negative staining in Normal tonsil tissue.

    • ab108984 showing negative staining in Normal placenta tissue.

    • ab108984 showing positive staining in Prostatic carcinoma tissue.

    • ab108984 showing negative staining in Breast carcinoma tissue.

    • ab108984 showing negative staining in Cervical carcinoma tissue.

    • ab108984 showing negative staining in Normal breast tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Prostatic Acid Phosphatase antibody [EPR4066] (ab108984)

    ab108984 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Prostate Cancer)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA based buffer pH 9.0
    Permeabilization No
    Specification Prostate Cancer
    Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Nov 13 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"