Anti-Prostatic Acid Phosphatase antibody [EPR4067] (ab109004)

Overview

  • Product nameAnti-Prostatic Acid Phosphatase antibody [EPR4067]
    See all Prostatic Acid Phosphatase primary antibodies
  • Description
    Rabbit monoclonal [EPR4067] to Prostatic Acid Phosphatase
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Prostatic Acid Phosphatase.

  • Positive control
    • Human prostate cancer lysate; Human prostatic normal and adenocarcinoma tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • Sequence similaritiesBelongs to the histidine acid phosphatase family.
    • Cellular localizationSecreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • Acid phosphatase prostate antibody
      • ACP 3 antibody
      • ACP3 antibody
      • acpP antibody
      • Ecto-5'-nucleotidase antibody
      • PAP antibody
      • PPAP_HUMAN antibody
      • Prostatic acid phosphatase antibody
      • Prostatic acid phosphotase antibody
      • Thiamine monophosphatase antibody
      • TMPase antibody
      see all

    Anti-Prostatic Acid Phosphatase antibody [EPR4067] images

    • Anti-Prostatic Acid Phosphatase antibody [EPR4067] (ab109004) at 1/1000 dilution + Prostate cancer lysate at 10 µg

      Predicted band size : 45 kDa
    • ab109004 at 1/250 dilution, staining Prostatic Acid Phosphatase in Human prostatic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109004 at 1/250 dilution, staining Prostatic Acid Phosphatase in Human prostatic tissue by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109004 showing negative staining in Breast carcinoma tissue.

    • ab109004 showing negative staining in Normal uterus tissue.

    • ab109004 showing positive staining in Prostate hyperplasia tissue.

    References for Anti-Prostatic Acid Phosphatase antibody [EPR4067] (ab109004)

    ab109004 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you very much for your reply.

    I hope this will resolve the problem - please keep us updated!

    I wish you a good day and look forward hearing back from you!

    Thank you for contacting us. I am very sorry to hear that you have been experiencing problems also with this antibody.

    I have looked through the protocol provided and compared it to the information we have. There are only two points which I w...

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    Thank you for your reply. I am sorry that the results with ab109004 were so poor. I am sending a free of charge vial of ab9319 on the order# ***, which should arrive tomorrow. Please keep me updated about the results with this replacement antibody, and...

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    Sure, I can send a vial of ab9319. Could you send me your order or PO number so I can find your original order?

    Thank you for your reply. I would be happy to send an antibody to replace the vial of ab109004, but unfortunately we don't have any other ACPP antibodies that have been validated in mouse. So we can not guarantee that another ACPP antibody will work wi...

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    Thank you for contacting us and letting us know about the trouble with this antibody. Ab109004 has tested positive in Western blot with murine lysate, but I do have a few additional questions so that I can help further. 1) Are there any non-specific ba...

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    The immunogen peptide maps between amino acids 325 and 355 of human Prostatic Acid Phosphatase (UniProt: P15309). The antibody is predicted to recognize both isoforms of ACPP, based on the immunogen. Please contact us if you have any other questions.

    Thank you for contacting us. This product has been tested successfully in human and mouse in WB, IP, IHC-P, ICC applications. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"